CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044495
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Clathrin heavy chain 1 
Protein Synonyms/Alias
  
Gene Name
 CLTC 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78AIMNPASKVIALKAGubiquitination[1]
146GDRCYDEKMYDAAKLubiquitination[2]
152EKMYDAAKLLYNNVSubiquitination[1, 2, 3, 4]
201CFACVDGKEFRLAQMubiquitination[2, 5]
284WSRVNIPKVLRAAEQubiquitination[1, 3]
329AWKEGQFKDIITKVAubiquitination[1]
334QFKDIITKVANVELYubiquitination[1]
378RAVNYFSKVKQLPLVacetylation[6, 7]
378RAVNYFSKVKQLPLVubiquitination[1, 2, 3, 4, 8]
380VNYFSKVKQLPLVKPubiquitination[2, 3]
386VKQLPLVKPYLRSVQacetylation[5, 6]
386VKQLPLVKPYLRSVQubiquitination[1, 2, 3, 5]
398SVQNHNNKSVNESLNubiquitination[1, 4]
438SLAQRLEKHELIEFRacetylation[5, 6, 7]
438SLAQRLEKHELIEFRubiquitination[1, 2, 3]
546VMKEYLTKVDKLDASubiquitination[1, 2, 3, 4, 5]
549EYLTKVDKLDASESLubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 612 AA 
Protein Sequence
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV VIIDMNDPSN 60
PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK ARESYVETEL IFALAKTNRL 120
AELEEFINGP NNAHIQQVGD RCYDEKMYDA AKLLYNNVSN FGRLASTLVH LGEYQAAVDG 180
ARKANSTRTW KEVCFACVDG KEFRLAQMCG LHIVVHADEL EELINYYQDR GYFEELITML 240
EAALGLERAH MGMFTELAIL YSKFKPQKMR EHLELFWSRV NIPKVLRAAE QAHLWAELVF 300
LYDKYEEYDN AIITMMNHPT DAWKEGQFKD IITKVANVEL YYRAIQFYLE FKPLLLNDLL 360
MVLSPRLDHT RAVNYFSKVK QLPLVKPYLR SVQNHNNKSV NESLNNLFIT EEDYQALRTS 420
IDAYDNFDNI SLAQRLEKHE LIEFRRIAAY LFKGNNRWKQ SVELCKKDSL YKDAMQYASE 480
SKDTELAEEL LQWFLQEEKR ECFGACLFTC YDLLRPDVVL ETAWRHNIMD FAMPYFIQVM 540
KEYLTKVDKL DASESLRKEE EQATETQPIV YGQPQLMLTA GPSVAVPPQA PFGYGYTAPP 600
YGQPQPGFGY SM 612 
Gene Ontology
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0030130; C:clathrin coat of trans-Golgi network vesicle; IEA:InterPro.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000547; Clathrin_H-chain/VPS_repeat.
 IPR016025; Clathrin_H-chain_link/propller.
 IPR001473; Clathrin_H-chain_propeller_N.
 IPR022365; Clathrin_H-chain_propeller_rpt.
 IPR011990; TPR-like_helical. 
Pfam
 PF00637; Clathrin
 PF01394; Clathrin_propel 
SMART
 SM00299; CLH 
PROSITE
  
PRINTS