CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011275
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-protein ligase E3A 
Protein Synonyms/Alias
 E6AP ubiquitin-protein ligase; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54 
Gene Name
 UBE3A 
Gene Synonyms/Alias
 E6AP; EPVE6AP; HPVE6A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
100KGARIDFKDVTYLTEubiquitination[1]
203QGDNNLQKLGPDDVSubiquitination[1]
327FQQLITYKVISNEFNubiquitination[1]
418VKTLDCRKPLIPFEEubiquitination[1]
445DKDYTFFKVETENKFubiquitination[1]
529MENPADLKKQLYVEFubiquitination[1]
701YSDYILNKSVEKQFKubiquitination[1]
779HSFTDEQKRLFLQFTubiquitination[1]
806KLKMIIAKNGPDTERubiquitination[1]
847LKAITYAKGFGML**ubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. 
Sequence Annotation
 DOMAIN 776 875 HECT.
 REGION 401 418 E6-binding.
 REGION 418 517 Interaction with HCV core protein.
 ACT_SITE 843 843 Glycyl thioester intermediate.
 MOD_RES 218 218 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; Host-virus interaction; Ligase; Nucleus; Phosphoprotein; Polymorphism; Proteasome; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 875 AA 
Protein Sequence
MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC 60
DPHPSKKGAS SAYLENSKGA PNNSCSEIKM NKKGARIDFK DVTYLTEEKV YEILELCRER 120
EDYSPLIRVI GRVFSSAEAL VQSFRKVKQH TKEELKSLQA KDEDKDEDEK EKAACSAAAM 180
EEDSEASSSR IGDSSQGDNN LQKLGPDDVS VDIDAIRRVY TRLLSNEKIE TAFLNALVYL 240
SPNVECDLTY HNVYSRDPNY LNLFIIVMEN RNLHSPEYLE MALPLFCKAM SKLPLAAQGK 300
LIRLWSKYNA DQIRRMMETF QQLITYKVIS NEFNSRNLVN DDDAIVAASK CLKMVYYANV 360
VGGEVDTNHN EEDDEEPIPE SSELTLQELL GEERRNKKGP RVDPLETELG VKTLDCRKPL 420
IPFEEFINEP LNEVLEMDKD YTFFKVETEN KFSFMTCPFI LNAVTKNLGL YYDNRIRMYS 480
ERRITVLYSL VQGQQLNPYL RLKVRRDHII DDALVRLEMI AMENPADLKK QLYVEFEGEQ 540
GVDEGGVSKE FFQLVVEEIF NPDIGMFTYD ESTKLFWFNP SSFETEGQFT LIGIVLGLAI 600
YNNCILDVHF PMVVYRKLMG KKGTFRDLGD SHPVLYQSLK DLLEYEGNVE DDMMITFQIS 660
QTDLFGNPMM YDLKENGDKI PITNENRKEF VNLYSDYILN KSVEKQFKAF RRGFHMVTNE 720
SPLKYLFRPE EIELLICGSR NLDFQALEET TEYDGGYTRD SVLIREFWEI VHSFTDEQKR 780
LFLQFTTGTD RAPVGGLGKL KMIIAKNGPD TERLPTSHTC FNVLLLPEYS SKEKLKERLL 840
KAITYAKGFG ML 852 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0003713; F:transcription coactivator activity; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; IBA:RefGenome.
 GO:0007420; P:brain development; TAS:ProtInc.
 GO:0001541; P:ovarian follicle development; IEA:Compara.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0060736; P:prostate gland growth; IEA:Compara.
 GO:0070936; P:protein K48-linked ubiquitination; IDA:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome.
 GO:0035037; P:sperm entry; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR000569; HECT.
 IPR017134; Ubiquitin-protein_ligase_E6-AP. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT 
PRINTS