CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044924
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-directed RNA polymerase 
Protein Synonyms/Alias
  
Gene Name
 POLRMT 
Gene Synonyms/Alias
 hCG_20561 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43GRPGLPGKEGTAGGVmethylation[1]
169PFQSGEFKALTRRLQubiquitination[2]
216LLQEAPGKLSLDVEQubiquitination[2]
417TLQCLFEKQLHMELAacetylation[3]
547ALQNHYRKYLCLLASubiquitination[3]
637VQQIGILKPHPAYVQacetylation[4]
1212RFCSEPQKILEASQLubiquitination[5]
Reference
 [1] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome; Transcription; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1245 AA 
Protein Sequence
MRRRGPVGWP GAACVMSALC WGRGAAGLKR ALRPCGRPGL PGKEGTAGGV CGPRRSSSAS 60
PQEQDQDRRK DWGHVELLEV LQARVRQLQA ESVSEVVVNR VDVARLPECG SGDGSLQPPR 120
KVQMGAKDAT PVPCGRWAKI LEKDKRTQQM RMQRLKAKLQ MPFQSGEFKA LTRRLQVEPR 180
LLSKQMAGCL EDCTRQAPES PWEEQLARLL QEAPGKLSLD VEQAPSGQHS QAQLSGQQQR 240
LLAFFKCCLL TDQLPLAHHL LVVHHGQRQK RKLLTLDMYN AVMLGWARQG AFKELVYVLF 300
MVKDAGLTPD LLSYAAALQC MGRQDQDAGT IERCLEQMSQ EGLKLQALFT AVLLSEEDRA 360
TVLKAVHKVK PTFSLPPQLP PPVNTSKLLR DVYAKDGRVS YPKLHLPLKT LQCLFEKQLH 420
MELASRVCVV SVEKPTLPSK EVKHARKTLK TLRDQWEKAL CRALRETKNR LEREVYEGRF 480
SLYPFLCLLD EREVVRMLLQ VLQALPAQGE SFTTLARELS ARTFSRHVVQ RQRVSGQVQA 540
LQNHYRKYLC LLASDAEVPE PCLPRQYWEE LGAPEALREQ PWPLPVQMEL GKLLAEMLVQ 600
ATQMPCSLDK PHRSSRLVPV LYHVYSFRNV QQIGILKPHP AYVQLLEKAA EPTLTFEAVD 660
VPMLCPPLPW TSPHSGAFLL SPTKLMRTVE GATQHQELLE TCPPTALHGA LDALTQLGNC 720
AWRVNGRVLD LVLQLFQAKG CPQLGVPAPP SEAPQPPEAH LPHSAAPARK AELRRELAHC 780
QKVAREMHSL RAEALYRLSL AQHLRDRVFW LPHNMDFRGR TYPCPPHFNH LGSDVARALL 840
EFAQGRPLGP HGLDWLKIHL VNLTGLKKRE PLRKRLAFAE EVMDDILDSA DQPLTGRKWW 900
MGAEEPWQTL ACCMEVANAV RASDPAAYVS HLPVHQDGSC NGLQHYAALG RDSVGAASVN 960
LEPSDVPQDV YSGVAAQVEV FRRQDAQRGM RVAQVLEGFI TRKVVKQTVM TVVYGVTRYG 1020
GRLQIEKRLR ELSDFPQEFV WEASHYLVRQ VFKSLQEMFS GTRAIQHWLT ESARLISHMG 1080
SVVEWVTPLG VPVIQPYRLD SKVKQIGGGI QSITYTHNGD ISRKPNTRKQ KNGFPPNFIH 1140
SLDSSHMMLT ALHCYRKGLT FVSVHDCYWT HAADVSVMNQ VCREQFVRLH SEPILQDLSR 1200
FLVKRFCSEP QKILEASQLK ETLQAVPKPG AFDLEQVKRS TYFFS 1245 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:InterPro. 
Interpro
 IPR024075; DNA-dir_RNA_pol_helix_hairpin.
 IPR002092; DNA-dir_Rpol_phage-type. 
Pfam
 PF00940; RNA_pol 
SMART
  
PROSITE
 PS00900; RNA_POL_PHAGE_1
 PS00489; RNA_POL_PHAGE_2 
PRINTS