CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013892
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2-oxoisovalerate dehydrogenase subunit beta 
Protein Synonyms/Alias
 Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta 
Gene Name
 TTHA0230 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 
NCBI Taxa ID
 300852 
Lysine Modification
Position
Peptide
Type
References
33VLGEDVGKRGGVFLVacetylation[1]
181KRLYRSVKEEVPEEDacetylation[1]
Reference
 [1] Acetylome with structural mapping reveals the significance of lysine acetylation in Thermus thermophilus.
 Okanishi H, Kim K, Masui R, Kuramitsu S.
 J Proteome Res. 2013 Aug 1;. [PMID: 23901841] 
Functional Description
 The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity). 
Sequence Annotation
 REGION 58 60 Thiamine pyrophosphate.
 REGION 83 86 Substrate.
 REGION 86 89 Thiamine pyrophosphate.
 ACT_SITE 129 129 Proton acceptor (Probable).
 BINDING 29 29 Thiamine pyrophosphate.
 BINDING 82 82 Thiamine pyrophosphate.
 BINDING 129 129 Substrate.  
Keyword
 3D-structure; Complete proteome; Oxidoreductase; Reference proteome; Thiamine pyrophosphate. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 324 AA 
Protein Sequence
MALMTMVQAL NRALDEEMAK DPRVVVLGED VGKRGGVFLV TEGLLQKYGP DRVMDTPLSE 60
AAIVGAALGM AAHGLRPVAE IQFADYIFPG FDQLVSQVAK LRYRSGGQFT APLVVRMPSG 120
GGVRGGHHHS QSPEAHFVHT AGLKVVAVST PYDAKGLLKA AIRDEDPVVF LEPKRLYRSV 180
KEEVPEEDYT LPIGKAALRR EGKDLTLIGY GTVMPEVLQA AAELAKAGVS AEVLDLRTLM 240
PWDYEAVMNS VAKTGRVVLV SDAPRHASFV SEVAATIAED LLDMLLAPPI RVTGFDTPYP 300
YAQDKLYLPT VTRILNAAKR ALDY 324 
Gene Ontology
 GO:0003863; F:3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity; IEA:EC. 
Interpro
 IPR009014; Transketo_C/Pyr-ferredox_oxred.
 IPR015941; Transketolase-like_C.
 IPR005475; Transketolase-like_Pyr-bd.
 IPR005476; Transketolase_C. 
Pfam
 PF02779; Transket_pyr
 PF02780; Transketolase_C 
SMART
 SM00861; Transket_pyr 
PROSITE
  
PRINTS