CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040295
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Dual-specificity mitogen-activated protein kinase kinase 7 
Protein Synonyms/Alias
  
Gene Name
 MAP2K7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AASSLEQKLSRLEAKubiquitination[1, 2, 3]
95ESIEIDQKLQEIMKQubiquitination[2, 4]
287GVIHRDVKPSNILLDubiquitination[2, 3, 4]
300LDERGQIKLCDFGISubiquitination[2]
368QFPYKNCKTDFEVLTubiquitination[2]
415RKRPKYNKLLEHSFIubiquitination[5]
442WFKDVMAKTESPRTSubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 461 AA 
Protein Sequence
MAASSLEQKL SRLEAKLKQE NREARRRIDL NLDISPQRPR PTLQLPLAND GGSRSPSSES 60
SPQHPTPPAR PRHMLGLPST LFTPRSMESI EIDQKLQEIM KQTGYLTIGG QVPPSLWRGE 120
GGGPARLDPS WERQWGAGGG GRPGTLQPSL SSQRYQAEIN DLENLGEMGS GTCGQVWKMR 180
FRKTGHVIAV KQMRRSGNKE ENKRILMDLD VVLKSHDCPY IVQCFGTFIT NTDVFIAMEL 240
MGTCAEKLKK RMQGPIPERI LGKMTVAIVK ALYYLKEKHG VIHRDVKPSN ILLDERGQIK 300
LCDFGISGRL VDSKAKTRSA GCAAYMAPER IDPPDPTKPD YDIRADVWSL GISLVELATG 360
QFPYKNCKTD FEVLTKVLQE EPPLLPGHMG FSGDFQSFVK DCLTKDHRKR PKYNKLLEHS 420
FIKRYETLEV DVASWFKDVM AKTESPRTSG VLSQPHLPFF R 461 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS