UniProt Accession

 DAK1_YEAST; P54838; D6VZA3 

Genbank Protein ID

 Z38114; BK006946 

Genbank Nucleotide ID

 CAA86250.1; DAA09827.1 

Protein Name

 Dihydroxyacetone kinase 1 

Protein Synonyms/Alias

 DHA kinase 1; Glycerone kinase 1; Triokinase 1; Triose kinase 1 

Gene Name

 DAK1 

Gene Synonyms/Alias

 YML070W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
178	YGLDGTAKVAKIIND	ubiquitination	[1, 2]
203	HCKVPGRKFESELNE	acetylation	[3]
203	HCKVPGRKFESELNE	ubiquitination	[2]
245	ISKYMLPKLLDPNDK	acetylation	[3]
552	STATFEAKFGRASYV	acetylation	[3]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde (By similarity). 

Sequence Annotation

 DOMAIN 7 353 DhaK.
 DOMAIN 386 582 DhaL.
 NP_BIND 415 418 ATP (By similarity).
 NP_BIND 459 460 ATP (By similarity).
 NP_BIND 514 515 ATP (By similarity).
 NP_BIND 567 569 ATP (By similarity).
 REGION 51 54 Substrate binding (By similarity).
 ACT_SITE 220 220 Tele-hemiaminal-histidine intermediate
 BINDING 103 103 Substrate (By similarity).
 BINDING 108 108 Substrate (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 5 5 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 512 512 Phosphoserine.  

Keyword

 Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Glycerol metabolism; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 584 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004371; F:glycerone kinase activity; IDA:SGD.
 GO:0050354; F:triokinase activity; IEA:EC.
 GO:0019588; P:anaerobic glycerol catabolic process; IEA:UniProtKB-UniPathway.
 GO:0019563; P:glycerol catabolic process; ISS:SGD. 

Interpro

 IPR004006; Dak1.
 IPR004007; Dak2.
 IPR012734; DhaK_ATP. 

Pfam

 PF02733; Dak1
 PF02734; Dak2 

SMART

  

PROSITE

 PS51481; DHAK
 PS51480; DHAL 

PRINTS