CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017710
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein DBF4 homolog B 
Protein Synonyms/Alias
 Activator of S phase kinase-like protein 1; ASK-like protein 1; Chiffon homolog B; Dbf4-related factor 1 
Gene Name
 DBF4B 
Gene Synonyms/Alias
 ASKL1; DRF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
203AASPRIRKEDSCQASubiquitination[1, 2]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. 
Sequence Annotation
 DOMAIN 43 133 BRCT.
 ZN_FING 294 343 DBF4-type.  
Keyword
 Alternative splicing; Cell cycle; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 615 AA 
Protein Sequence
MMMHVQQLSL ASLCVKKQQP KKPEGTCPAA ESRTRKVARL KAPFLKIEDE SRKFRPFHHQ 60
FKSFPEISFL GPKDASPFEA PTTLGSMHHT RESKDGEPSP RSAAHTMPRR KKGYCECCQE 120
AFEELHVHLQ SAQHRSFALE AHLYAEVDRI IAQLSHSFAD IPFQAGLPRW SGSPASDCDP 180
LCPETLHPHQ PSHPRAASPR IRKEDSCQAS GIPEQDGTVD STQAPAERAG TGEVPGPIAS 240
CQDLGVSVDV FVDPPGIPVS RSPACQCLLP SSGFMELSSG PDLALFGHKR KVQFPSGSAK 300
KRVGASWPQA SFFVPIAPNP CGTRTTSGKR LPSLPLTGHE SRLLASLQPL CHSQTCLSLP 360
DPFPWQPTDR PAEFWATQPS WLGKGWPPGP EDSECTATGP VSQEAGQLLS CPTAPGWPSA 420
PLYSATSVQP SGAPVESRST SLLQPLPASA GASCSRCLWA PQPLQVPCLP VSQPWSQPQP 480
QPQPHAGREL LLRVPKVLGS SQGQAAPD 508 
Gene Ontology
 GO:0016235; C:aggresome; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW. 
Interpro
 IPR001357; BRCT_dom.
 IPR006572; Znf_DBF. 
Pfam
 PF07535; zf-DBF 
SMART
 SM00586; ZnF_DBF 
PROSITE
 PS50172; BRCT
 PS51265; ZF_DBF4 
PRINTS