CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043210
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 tRNA (cytosine(34)-C(5))-methyltransferase 
Protein Synonyms/Alias
  
Gene Name
 Nsun2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
46PEIVKENKLFEHYYQubiquitination[1]
109RKSPLLAKFHQFLVSubiquitination[1]
251KNIDVWKKWTTLNSLacetylation[2]
330MPGVSQWKVMTRDGQubiquitination[1]
410RQPKVQNKSAEAREPacetylation[3]
550KVINTGIKVWCRNNSacetylation[2, 3, 4]
604QENPFFRKLSSEAYSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MGRRARGRRF QQPPQPEGEE DASDGGRKRG QAGWEGGYPE IVKENKLFEH YYQELKIVPE 60
GEWDQFMESL REPLPATLRI TGYKRYPEEL AWHTNLSRKI LRKSPLLAKF HQFLVSETES 120
GNISRQEAVS MIPPLLLNVE PHHKILDMCA APGSKTTQLI EMLHADMSVP FPEGFVIAND 180
VDNKRCYLLV HQAKRLSSPC IMVVNHDASS IPRLTVDVDG RKEILFYDRI LCDVPCSGDG 240
TMRKNIDVWK KWTTLNSLQL HGLQLRIATR GAEQLAEGGR MVYSTCSLNP VEDEAVIAAL 300
LEKSEGALEL ADVSAELPGL KWMPGVSQWK VMTRDGQWFA DWHEVPQGRH TQIRPTMFPP 360
TDLEKLQAMH LERCLRILPH HQNTGGFFVA VLVKKAPMPW NKRQPKVQNK SAEAREPRVS 420
SHVAATEGNP SDQSELESQM ITGAGDSETA HNTENTESNE KKDGVCGPPP SKKMKLFGFK 480
EDPFVFIPED DPLFPPIEKF YALDPSFPRM NLLTRTTEGK KRQLYMVSKE LRNVLLNNSE 540
KMKVINTGIK VWCRNNSGEE FDCAFRLAQE GIYTLYPFIN SRIITVSMED VKTLLTQENP 600
FFRKLSSEAY SQVKDLAKGS VVLKYEPDSA NPDTLQCPIV LCGWRGKASI RTFVPKNERL 660
HYLRMMGLEV LGEKKKEGVI LTNENAASPE QPGDEDAKQT AQDPCVPDSV PGCDAAAAEP 720
SR 722 
Gene Ontology
 GO:0016428; F:tRNA (cytosine-5-)-methyltransferase activity; IEA:InterPro. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR023267; RCMT.
 IPR023270; RCMT_NCL1. 
Pfam
 PF01189; Nol1_Nop2_Fmu 
SMART
  
PROSITE
 PS51606; SAM_MT_TRM4 
PRINTS
 PR02008; RCMTFAMILY.
 PR02011; RCMTNCL1.