UniProt Accession

 OTUL_MOUSE; Q3UCV8; Q3UY59; Q5M8N1; Q8R027 

Genbank Protein ID

 AK134955; AK150371; GL456173; BC028541; BC087945 

Genbank Nucleotide ID

 BAE22354.1; BAE29504.1; AAH28541.1; AAH87945.1 

Protein Name

 Ubiquitin thioesterase otulin 

Protein Synonyms/Alias

 Deubiquitinating enzyme otulin; OTU domain-containing deubiquitinase with linear linkage specificity; Ubiquitin thioesterase Gumby 

Gene Name

 Fam105b 

Gene Synonyms/Alias

 Gum 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	ADEIEKEKELLIHER	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Deubiquitinase that specifically removes linear ('M-1'- linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis. Associates with the LUBAC complex via direct interaction with RNF31 and counteracts its action by cleaving linear polyubiquitin chains to substrates. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by counteracting activity of the LUBAC complex. 

Sequence Annotation

 DOMAIN 118 346 OTU.
 REGION 95 96 Linear diubiquitin binding (By
 REGION 124 126 Linear diubiquitin binding (By
 REGION 255 259 Linear diubiquitin binding (By
 REGION 283 289 Linear diubiquitin binding (By
 REGION 336 338 Linear diubiquitin binding (By
 MOTIF 349 352 PDZ-binding.
 ACT_SITE 126 126
 ACT_SITE 129 129 Nucleophile.
 ACT_SITE 339 339
 BINDING 314 314 Linear diubiquitin.  

Keyword

 Acetylation; Angiogenesis; Coiled coil; Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 352 AA 

Protein Sequence

Gene Ontology

  

Interpro

 IPR023235; FAM105.
 IPR023237; FAM105B.
 IPR019400; Peptidase_C65_otubain. 

Pfam

 PF10275; Peptidase_C65 

SMART

  

PROSITE

 PS50802; OTU 

PRINTS

 PR02055; PROTEINF105.
 PR02057; PROTEINF105B.