CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CLIP-associating protein 2 
Protein Synonyms/Alias
 Cytoplasmic linker-associated protein 2; Protein Orbit homolog 2; hOrbit2 
Gene Name
 CLASP2 
Gene Synonyms/Alias
 KIAA0627 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
671SSNWSERKEGLLGLQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. 
Sequence Annotation
 REPEAT 173 208 HEAT 1.
 REPEAT 209 245 HEAT 2.
 REPEAT 772 809 HEAT 3.
 REPEAT 1098 1135 HEAT 4.
 REPEAT 1216 1253 HEAT 5.
 REGION 444 580 Interaction with microtubules, MAPRE1 and
 REGION 872 1294 Interaction with RSN and localization to
 REGION 1017 1294 Required for cortical localization.
 MOTIF 494 497 Microtubule tip localization signal.
 MOTIF 517 520 Microtubule tip localization signal.
 MOD_RES 8 8 Phosphoserine.
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 455 455 Phosphoserine.
 MOD_RES 507 507 Phosphoserine (By similarity).
 MOD_RES 525 525 Phosphoserine.
 MOD_RES 529 529 Phosphoserine.
 MOD_RES 596 596 Phosphoserine.
 MOD_RES 1029 1029 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Cell membrane; Cell projection; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; Kinetochore; Membrane; Microtubule; Mitosis; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1294 AA 
Protein Sequence
MAMGDDKSFD DEESVDGNRP SSAASAFKVP APKTSGNPAN SARKPGSAGG PKVGGASKEG 60
GAGAVDEDDF IKAFTDVPSI QIYSSRELEE TLNKIREILS DDKHDWDQRA NALKKIRSLL 120
VAGAAQYDCF FQHLRLLDGA LKLSAKDLRS QVVREACITV AHLSTVLGNK FDHGAEAIVP 180
TLFNLVPNSA KVMATSGCAA IRFIIRHTHV PRLIPLITSN CTSKSVPVRR RSFEFLDLLL 240
QEWQTHSLER HAAVLVETIK KGIHDADAEA RVEARKTYMG LRNHFPGEAE TLYNSLEPSY 300
QKSLQTYLKS SGSVASLPQS DRSSSSSQES LNRPFSSKWS TANPSTVAGR VSAGSSKASS 360
LPGSLQRSRS DIDVNAAAGA KAHHAAGQSV RRGRLGAGAL NAGSYASLED TSDKLDGTAS 420
EDGRVRAKLS APLAGMGNAK ADSRGRSRTK MVSQSQPGSR SGSPGRVLTT TALSTVSSGV 480
QRVLVNSASA QKRSKIPRSQ GCSREASPSR LSVARSSRIP RPSVSQGCSR EASRESSRDT 540
SPVRSFQPLA SRHHSRSTGA LYAPEVYGAS GPGYGISQSS RLSSSVSAMR VLNTGSDVEE 600
AVADALKKPA RRRYESYGMH SDDDANSDAS SACSERSYSS RNGSIPTYMR QTEDVAEVLN 660
RCASSNWSER KEGLLGLQNL LKNQRTLSRV ELKRLCEIFT RMFADPHGKR VFSMFLETLV 720
DFIQVHKDDL QDWLFVLLTQ LLKKMGADLL GSVQAKVQKA LDVTRESFPN DLQFNILMRF 780
TVDQTQTPSL KVKVAILKYI ETLAKQMDPG DFINSSETRL AVSRVITWTT EPKSSDVRKA 840
AQSVLISLFE LNTPEFTMLL GALPKTFQDG ATKLLHNHLR NTGNGTQSSM GSPLTRPTPR 900
SPANWSSPLT SPTNTSQNTL SPSAFDYDTE NMNSEDIYSS LRGVTEAIQN FSFRSQEDMN 960
EPLKRDSKKD DGDSMCGGPG MSDPRAGGDA TDSSQTALDN KASLLHSMPT HSSPRSRDYN 1020
PYNYSDSISP FNKSALKEAM FDDDADQFPD DLSLDHSDLV AELLKELSNH NERVEERKIA 1080
LYELMKLTQE ESFSVWDEHF KTILLLLLET LGDKEPTIRA LALKVLREIL RHQPARFKNY 1140
AELTVMKTLE AHKDPHKEVV RSAEEAASVL ATSISPEQCI KVLCPIIQTA DYPINLAAIK 1200
MQTKVIERVS KETLNLLLPE IMPGLIQGYD NSESSVRKAC VFCLVAVHAV IGDELKPHLS 1260
QLTGSKMKLL NLYIKRAQTG SGGADPTTDV SGQS 1294 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:MGI.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005881; C:cytoplasmic microtubule; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0005828; C:kinetochore microtubule; TAS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0008107; F:galactoside 2-alpha-L-fucosyltransferase activity; NAS:UniProtKB.
 GO:0051010; F:microtubule plus-end binding; IDA:UniProtKB.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007163; P:establishment or maintenance of cell polarity; TAS:UniProtKB.
 GO:0034453; P:microtubule anchoring; IMP:UniProtKB.
 GO:0007020; P:microtubule nucleation; IMP:UniProtKB.
 GO:0031023; P:microtubule organizing center organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0007026; P:negative regulation of microtubule depolymerization; NAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR024395; CLASP_N_dom. 
Pfam
 PF12348; CLASP_N 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS