UniProt Accession

 PEPD_HUMAN; P12955; A8K3Z1; A8K416; A8K696; A8MX47; B4DDB7; B4DGJ1; E9PCE8; Q8TBN9; Q9BT75 

Genbank Protein ID

 J04605; BT006692; AK290756; AK290781; AK291561; AK293126; AK294619; AC008744; AC010485; BC004305; BC015027; BC028295 

Genbank Nucleotide ID

 AAA60064.1; AAP35338.1; BAF83445.1; BAF83470.1; BAF84250.1; BAG56678.1; BAG57802.1; AAH04305.1; AAH15027.1; AAH28295.1 

Protein Name

 Xaa-Pro dipeptidase 

Protein Synonyms/Alias

 X-Pro dipeptidase; Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase 

Gene Name

 PEPD 

Gene Synonyms/Alias

 PRD 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	RLCERLRKNPAVQAG	ubiquitination	[1]
303	GKFTADQKAVYEAVL	ubiquitination	[2]
484	ACMAGCDKAFTPFSG	ubiquitination	[3]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. 

Sequence Annotation

 METAL 276 276 Manganese 1.
 METAL 287 287 Manganese 1.
 METAL 287 287 Manganese 2.
 METAL 370 370 Manganese 2.
 METAL 412 412 Manganese 2.
 METAL 452 452 Manganese 1.
 METAL 452 452 Manganese 2.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Collagen degradation; Complete proteome; Dipeptidase; Direct protein sequencing; Disease mutation; Hydrolase; Manganese; Metal-binding; Metalloprotease; Polymorphism; Protease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 493 AA 

Protein Sequence

Gene Ontology

 GO:0004177; F:aminopeptidase activity; IEA:InterPro.
 GO:0016805; F:dipeptidase activity; IEA:UniProtKB-KW.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0004181; F:metallocarboxypeptidase activity; TAS:ProtInc.
 GO:0006520; P:cellular amino acid metabolic process; TAS:ProtInc.
 GO:0030574; P:collagen catabolic process; IEA:UniProtKB-KW.
 GO:0006508; P:proteolysis; TAS:ProtInc. 

Interpro

 IPR007865; Aminopep_P_N.
 IPR000994; Pept_M24_structural-domain.
 IPR001131; Peptidase_M24B_aminopep-P_CS. 

Pfam

 PF05195; AMP_N
 PF00557; Peptidase_M24 

SMART

 SM01011; AMP_N 

PROSITE

 PS00491; PROLINE_PEPTIDASE 

PRINTS