CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007008
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosome-associated complex subunit SSZ1 
Protein Synonyms/Alias
 DnaK-related protein SSZ1; Heat shock protein 70 homolog SSZ1; Pleiotropic drug resistance protein 13 
Gene Name
 SSZ1 
Gene Synonyms/Alias
 PDR13; YHR064C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
85FDKCDVSKCANGAPAubiquitination[1]
365EILGPQNKNASNNPNubiquitination[1]
410VNTPHLKKPIGLIGAubiquitination[1]
490EPEVVREKLYTLGTKacetylation[2]
490EPEVVREKLYTLGTKubiquitination[1]
535LKTGNAVKGEL****ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. SSZ1 is required for ZUO1 to function efficiently as a J-protein for SSB1/SSB2. Also involved in pleiotropic drug resistance by post-translational activation of transcription factor PDR1. 
Sequence Annotation
 REGION 400 538 Peptide-binding domain (Potential).
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 480 480 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 538 AA 
Protein Sequence
MSSPVIGITF GNTSSSIAYI NPKNDVDVIA NPDGERAIPS ALSYVGEDEY HGGQALQQLI 60
RNPKNTIINF RDFIGLPFDK CDVSKCANGA PAVEVDGKVG FVISRGEGKE EKLTVDEVVS 120
RHLNRLKLAA EDYIGSAVKE AVLTVPTNFS EEQKTALKAS AAKIGLQIVQ FINEPSAALL 180
AHAEQFPFEK DVNVVVADFG GIRSDAAVIA VRNGIFTILA TAHDLSLGGD NLDTELVEYF 240
ASEFQKKYQA NPRKNARSLA KLKANSSITK KTLSNATSAT ISIDSLADGF DYHASINRMR 300
YELVANKVFA QFSSFVDSVI AKAELDPLDI DAVLLTGGVS FTPKLTTNLE YTLPESVEIL 360
GPQNKNASNN PNELAASGAA LQARLISDYD ADELAEALQP VIVNTPHLKK PIGLIGAKGE 420
FHPVLLAETS FPVQKKLTLK QAKGDFLIGV YEGDHHIEEK TLEPIPKEEN AEEDDESEWS 480
DDEPEVVREK LYTLGTKLME LGIKNANGVE IIFNINKDGA LRVTARDLKT GNAVKGEL 538 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; IGI:SGD.
 GO:0002181; P:cytoplasmic translation; IGI:SGD.
 GO:0006450; P:regulation of translational fidelity; IMP:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD. 
Interpro
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.