UniProt Accession

 AT1A3_RAT; P06687; Q16732; Q9Z1G6 

Genbank Protein ID

 M14513; X05883; M28648 

Genbank Nucleotide ID

 AAA40777.1; CAA29307.1; AAA41672.1 

Protein Name

 Sodium/potassium-transporting ATPase subunit alpha-3 

Protein Synonyms/Alias

 Na(+)/K(+) ATPase alpha-3 subunit; Na(+)/K(+) ATPase alpha(III) subunit; Sodium pump subunit alpha-3 

Gene Name

 Atp1a3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
26	RRDLDDLKKEVAMTE	acetylation	[1]
26	RRDLDDLKKEVAMTE	ubiquitination	[2]
27	RDLDDLKKEVAMTEH	ubiquitination	[2]
57	VQGLTHSKAQEILAR	acetylation	[1]
146	YQEAKSSKIMESFKN	ubiquitination	[2, 3]
152	SKIMESFKNMVPQQA	ubiquitination	[2, 3]
202	IISAHGCKVDNSSLT	ubiquitination	[3]
424	LCNRAVFKGGQDNIP	acetylation	[1]
424	LCNRAVFKGGQDNIP	ubiquitination	[2]
434	QDNIPVLKRDVAGDA	acetylation	[1]
434	QDNIPVLKRDVAGDA	ubiquitination	[2]
448	ASESALLKCIELSSG	ubiquitination	[3]
458	ELSSGSVKLMRERNK	ubiquitination	[3, 4]
477	IPFNSTNKYQLSIHE	acetylation	[1]
595	AVPDAVGKCRSAGIK	ubiquitination	[3]
602	KCRSAGIKVIMVTGD	ubiquitination	[3]
615	GDHPITAKAIAKGVG	ubiquitination	[2]
619	ITAKAIAKGVGIISE	ubiquitination	[2]
688	ARTSPQQKLIIVEGC	ubiquitination	[3]
716	VNDSPALKKADIGVA	methylation	[5]
716	VNDSPALKKADIGVA	ubiquitination	[2]
717	NDSPALKKADIGVAM	methylation	[5]
717	NDSPALKKADIGVAM	ubiquitination	[2]
763	RLIFDNLKKSIAYTL	methylation	[5]
763	RLIFDNLKKSIAYTL	ubiquitination	[2, 3]
764	LIFDNLKKSIAYTLT	methylation	[5]
764	LIFDNLKKSIAYTLT	ubiquitination	[3]
833	PRNPRTDKLVNERLI	ubiquitination	[2, 3]
940	SVFQQGMKNKILIFG	ubiquitination	[3]
1009	NPGGWVEKETYY***	acetylation	[1]
1009	NPGGWVEKETYY***	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561] 

Functional Description

 This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. 

Sequence Annotation

 REGION 72 74 Interaction with phosphoinositide-3
 ACT_SITE 366 366 4-aspartylphosphate intermediate (By
 METAL 707 707 Magnesium (By similarity).
 METAL 711 711 Magnesium (By similarity).
 MOD_RES 265 265 Phosphoserine (By similarity).
 MOD_RES 368 368 Phosphothreonine (By similarity).
 MOD_RES 493 493 Phosphotyrosine (By similarity).
 MOD_RES 548 548 Phosphotyrosine (By similarity).
 MOD_RES 549 549 Phosphotyrosine (By similarity).
 MOD_RES 933 933 Phosphoserine; by PKA (By similarity).  

Keyword

 ATP-binding; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Potassium transport; Reference proteome; Sodium; Sodium transport; Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1013 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IDA:RGD.
 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0043209; C:myelin sheath; IDA:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005890; C:sodium:potassium-exchanging ATPase complex; IDA:RGD.
 GO:0045202; C:synapse; IDA:UniProtKB.
 GO:0005524; F:ATP binding; TAS:RGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005391; F:sodium:potassium-exchanging ATPase activity; ISS:UniProtKB.
 GO:0008344; P:adult locomotory behavior; IEA:Compara.
 GO:0006754; P:ATP biosynthetic process; IEA:InterPro.
 GO:0015991; P:ATP hydrolysis coupled proton transport; TAS:RGD.
 GO:0035235; P:ionotropic glutamate receptor signaling pathway; IEA:Compara.
 GO:0007613; P:memory; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0008542; P:visual learning; IEA:Compara. 

Interpro

 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR005775; ATPase_P-typ_Na/K_IIC.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 

Pfam

 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 

SMART

 SM00831; Cation_ATPase_N 

PROSITE

 PS00154; ATPASE_E1_E2 

PRINTS

 PR00119; CATATPASE.