CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024240
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glyceraldehyde-3-phosphate dehydrogenase 
Protein Synonyms/Alias
 GAPDH 
Gene Name
 gapA 
Gene Synonyms/Alias
 gap; MSMEG_3084; MSMEI_3006 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
257DLTAELAKSASVEDIpupylation[1, 2]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
  
Sequence Annotation
 NP_BIND 12 13 NAD (By similarity).
 REGION 158 160 Glyceraldehyde 3-phosphate binding (By
 REGION 217 218 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 159 159 Nucleophile (By similarity).
 BINDING 40 40 NAD (By similarity).
 BINDING 85 85 NAD; via carbonyl oxygen (By similarity).
 BINDING 189 189 Glyceraldehyde 3-phosphate (By
 BINDING 204 204 Glyceraldehyde 3-phosphate (By
 BINDING 240 240 Glyceraldehyde 3-phosphate (By
 BINDING 321 321 NAD (By similarity).
 CROSSLNK 257 257 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 Complete proteome; Cytoplasm; Glycolysis; Isopeptide bond; NAD; Oxidoreductase; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 340 AA 
Protein Sequence
MTIRVGVNGF GRIGRNFYRA LATQKAEGKN TDIEIVAVND LTDNATLAHL LKFDSILGRL 60
PQDVSLEGDD TIVIGDTKIK ALEVKEGPAA LPWGDLGVDV VVESTGIFTN AAKAKGHLDA 120
GAKKVIISAP ATDEDITIVL GVNDDKYDGS QNIISNASCT TNCLGPLAKV LNDEFGIVKG 180
LMTTIHAYTQ DQNLQDGPHK DLRRARAAAL NIVPTSTGAA KAIGLVLPEL KGKLDGYALR 240
VPIPTGSVTD LTAELAKSAS VEDINAAMKA AAEGPLKGIL KYYDAPIVSS DIVTDPHSSL 300
YDAGLTKVID NQAKVVSWYD NEWGYSNRLA DLVALVGKSL 340 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 
SMART
 SM00846; Gp_dh_N 
PROSITE
 PS00071; GAPDH 
PRINTS
 PR00078; G3PDHDRGNASE.