UniProt Accession

 IF5_MOUSE; P59325 

Genbank Protein ID

 BC039275; BC042622 

Genbank Nucleotide ID

 AAH39275.1 

Protein Name

 Eukaryotic translation initiation factor 5 

Protein Synonyms/Alias

 eIF-5 

Gene Name

 Eif5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
55	RPPTYPTKYFGCELG	ubiquitination	[1]
95	MLDGFIKKFVLCPEC	acetylation	[2]
143	KLCTFILKNPPENSD	acetylation	[3]
143	KLCTFILKNPPENSD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). 

Sequence Annotation

 DOMAIN 231 390 W2.
 NP_BIND 27 34 GTP (Potential).
 MOD_RES 10 10 Phosphoserine (By similarity).
 MOD_RES 227 227 Phosphoserine (By similarity).
 MOD_RES 387 387 Phosphoserine.
 MOD_RES 388 388 Phosphoserine.
 MOD_RES 417 417 Phosphoserine (By similarity).  

Keyword

 Complete proteome; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 429 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 

Interpro

 IPR016024; ARM-type_fold.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR002735; Transl_init_fac_IF2/IF5.
 IPR016189; Transl_init_fac_IF2/IF5_N.
 IPR016190; Transl_init_fac_IF2/IF5_Zn-bd.
 IPR003307; W2_domain. 

Pfam

 PF01873; eIF-5_eIF-2B
 PF02020; W2 

SMART

 SM00653; eIF2B_5
 SM00515; eIF5C 

PROSITE

 PS51363; W2 

PRINTS