| Tag | Content |
|---|
| CPLM ID | CPLM-015241 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Probable E3 ubiquitin-protein ligase HERC4 |
Protein Synonyms/Alias | HECT domain and RCC1-like domain-containing protein 4 |
Gene Name | Herc4 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 34 | RSDFFVNKKVRDVGC | ubiquitination | [1] | | 237 | RYVPNLLKSLRSQKI | ubiquitination | [1] | | 469 | AARLLFHKLIQPDHP | ubiquitination | [1] | | 488 | QVAASLEKNLIPKLT | ubiquitination | [1] | | 493 | LEKNLIPKLTSSLPD | ubiquitination | [1] | | 606 | ILHRVNEKTGQLIQY | ubiquitination | [1] | | 835 | KPSLDDLKELMPAVG | ubiquitination | [1] | | 959 | LEKNTEYKGEYWADH | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. |
Sequence Annotation | REPEAT 1 51 RCC1 1.
REPEAT 52 101 RCC1 2.
REPEAT 102 154 RCC1 3.
REPEAT 156 207 RCC1 4.
REPEAT 208 259 RCC1 5.
REPEAT 261 311 RCC1 6.
REPEAT 313 366 RCC1 7.
DOMAIN 730 1057 HECT.
ACT_SITE 1025 1025 Glycyl thioester intermediate (By |
Keyword | Alternative splicing; Complete proteome; Cytoplasm; Differentiation; Ligase; Reference proteome; Repeat; Spermatogenesis; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1057 AA |
Protein Sequence | MLCWGNASYG QLGLGGIDEE IVLEPRRSDF FVNKKVRDVG CGLRHTVFVL DDGTVYTCGC 60
NDLGQLGHEK SRKKPEQVVA LDAQNIVAVA CGEAHTLALN DKGQVYAWGL DSDGQLGLQG 120
SEECIRVPRN IKSLSDIQIV QVACGYYHSL ALSKASEVFC WGQNKYGQLG LGIDCQKQTS 180
PQLIKSLLGI PFMQVAAGGA HSFVLTLSGA IFGWGRNKFG QLGLNDENDR YVPNLLKSLR 240
SQKIVYICCG EDHTAALTKE GGVFTFGAGG YGQLGHNSTS HEINPRKVFE LMGSIVTQVA 300
CGRQHTSAFV PSSGRIYSFG LGGNGQLGTG STSNRKSPFT VKGNWFSYNG QCPQDIGSED 360
YFCVKRIFSG GDQSFSHYSS PQNCGPPDDF RCSDPSKQIW TVNEALIQKW LSYPSGRFPV 420
EIANEIDGTF SSSGCLNGSF LAISNDDHYR TGTRFSGVDM NAARLLFHKL IQPDHPQISQ 480
QVAASLEKNL IPKLTSSLPD VEALRFYLTL PECPLMSDCN NFTTIAIPFG TALVNLEKAP 540
LKVLENWWSV LEPPLFLKIV ELFKEVVVHL LKLYKIGIPP SERRIFNSFL HTALKVLEIL 600
HRVNEKTGQL IQYDKFYIHE VQELIDIRND YINWVQQQAY GVDVSHGVTE LADIPVTICT 660
YPFVFDAQAK TTLLQTDAVL QMQMAIDQAH RQNVSSLFLP VIESVNPCLI LVVRRENIVG 720
DAMEVLRKTK NIDYKKPLKV IFVGEDAVDA GGVRKEFFLL IMRELLDPKY GMFRYYEDSR 780
LIWFSDKTFE DSDLFHLIGV ICGLAIYNFT IVDLHFPLAL YKKLLKRKPS LDDLKELMPA 840
VGRSMQQLLD YPEDDIEETF CLNFTITVEN FGATEVKELV LNGADTAVNR QNRQEFVDAY 900
VDYIFNKSVA SLFDAFHAGF HKVCGGKVLL LFQPNELQAM VIGNTNYDWK ELEKNTEYKG 960
EYWADHPTIK IFWEVFHELP LEKKKQFLLF LTGSDRIPIL GMKSLKLVIQ STGGGESYLP 1020
VSHTCFNLLD LPKYTEKETL RCKLIQAIDH NEGFSLI 1057 |
Gene Ontology | GO:0005737; C:cytoplasm; IBA:RefGenome. GO:0005829; C:cytosol; IEA:UniProtKB-SubCell. GO:0005634; C:nucleus; IBA:RefGenome. GO:0004842; F:ubiquitin-protein ligase activity; TAS:UniProtKB. GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. GO:0007283; P:spermatogenesis; IMP:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |