CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029820
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Rho guanine nucleotide exchange factor 7 
Protein Synonyms/Alias
  
Gene Name
 ARHGEF7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
64SNYVREVKASEKPVSubiquitination[1]
73SEKPVSPKSGTLKSPubiquitination[1]
227VLTTGLSKPFMRLDKubiquitination[2]
240DKYPTLLKELERHMEubiquitination[2]
264QKSMAAFKNLSAQCQubiquitination[2]
524DEEFASRKSTAALEEubiquitination[2, 3]
547EAYCTSAKTRQTLNSubiquitination[2, 4]
617GSTSRSRKESAPQVLubiquitination[2]
630VLLPEEEKIIVEETKubiquitination[2, 3]
637KIIVEETKSNGQTVIubiquitination[2, 3, 4]
657VDTVYALKDEVQELRubiquitination[1, 2, 3, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 705 AA 
Protein Sequence
MTDNSNNQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTLNG RTGWFPSNYV 60
REVKASEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN ILETENEYSK ELQTVLSTYL 120
RPLQTSEKLS SANISYLMGN LEEICSFQQM LVQSLEECTK LPEAQQRVGG CFLNLMPQMK 180
TLYLTYCANH PSAVNVLTEH SEELGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK 240
ELERHMEDYH TDRQDIQKSM AAFKNLSAQC QEVRKRKELE LQILTEAIRN WEGDDIKTLG 300
NVTYMSQVLI QCAGSEEKNE RYLLLFPNVL LMLSASPRMS GFIYQGKLPT TGMTITKLED 360
SENHRNAFEI SGSMIERILV SCNNQQDLQE WVEHLQKQTK VTSVGNPTIK PHSVPSHTLP 420
SHPVTPSSKH ADSKPAPLTP AYHTLPHPSH HGTPHTTINW GPLEPPKTPK PWSLSCLRPA 480
PPLRPSAALC YKEDLSKSPK TMKKLLPKRK PERKPSDEEF ASRKSTAALE EDAQILKVIE 540
AYCTSAKTRQ TLNSTWQGTD LMHNHVLADD DQPSLDSLGR RSSLSRLEPS DLSEDSDYDS 600
IWTAHSYRMG STSRSRKESA PQVLLPEEEK IIVEETKSNG QTVIEEKSLV DTVYALKDEV 660
QELRQDNKKM KKSLEEEQRA RKDLEKLVRK VLKNMNDPAW DETNL 705 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2 
SMART
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.