UniProt Accession

 HOG1_YEAST; P32485; D6VYB1; Q06232; Q12294 

Genbank Protein ID

 L06279; U53878; Z73285; X89514; BK006945 

Genbank Nucleotide ID

 AAA34680.1; AAB67558.1; CAA97680.1; CAA61691.1; DAA09427.1 

Protein Name

 Mitogen-activated protein kinase HOG1 

Protein Synonyms/Alias

 MAP kinase HOG1; High osmolarity glycerol response protein 1 

Gene Name

 HOG1 

Gene Synonyms/Alias

 SSK3; YLR113W; L2931; L9354.2 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
146	GVIHRDLKPSNILIN	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription via the stress response element (STRE) in promoters of target genes. Upon osmotic shock, associates with the SKO1-SSN6-TUP1 complex, phosphorylates SKO1, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. Activates the SMP1 transcription factor and the RCK2 kinase, both also involved in the regulation of the expression of a subset of osmotic stress-related genes. Phosphorylation of HSL1 by HOG1 leads to a G2 arrest essential for cell survival at high osmolarity. Mediates also cell-cycle arrest in G1 phase by the dual targeting of SIC1. Regulates MFA2 ARE-mediated translation in response to carbon source. Targets RDP3 histone deacetylase to osmoresponsive promoters to induce gene expression on stress. Plays an essential role in maintaining water homeostasis, arsenite detoxification, copper-resistance, hydrogen peroxide response, adaptation to citric acid stress, and repression of the mating pathway activity. Required for the Golgi apparatus localization of MNN1. 

Sequence Annotation

 DOMAIN 23 302 Protein kinase.
 NP_BIND 29 37 ATP (By similarity).
 MOTIF 174 176 TXY.
 ACT_SITE 144 144 Proton acceptor (By similarity).
 BINDING 52 52 ATP (By similarity).
 MOD_RES 174 174 Phosphothreonine.
 MOD_RES 176 176 Phosphotyrosine.  

Keyword

 Activator; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 435 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004707; F:MAP kinase activity; IDA:SGD.
 GO:0034605; P:cellular response to heat; IMP:SGD.
 GO:0006972; P:hyperosmotic response; IMP:SGD.
 GO:0046020; P:negative regulation of transcription from RNA polymerase II promoter by pheromones; IEP:SGD.
 GO:0007231; P:osmosensory signaling pathway; IMP:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0046685; P:response to arsenic-containing substance; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR003527; MAP_kinase_CS.
 IPR008352; MAPK_p38.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS01351; MAPK
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS

 PR01773; P38MAPKINASE.