CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006289
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome P450 2F2 
Protein Synonyms/Alias
 CYPIIF2; Cytochrome P450-NAH-2; Naphthalene dehydrogenase; Naphthalene hydroxylase 
Gene Name
 Cyp2f2 
Gene Synonyms/Alias
 Cyp2f-2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
35GQLPPGPKPLPILGNubiquitination[1]
57DLLTSLTKLSKEYGSubiquitination[1]
91VKEALVDKGEEFSGRubiquitination[1]
139LRNFGMGKRSIEERIubiquitination[1]
159FLLEVLRKMEGKPFDubiquitination[1]
163VLRKMEGKPFDPVFIacetylation[2, 3, 4]
163VLRKMEGKPFDPVFIubiquitination[1]
242FRNFGGMKDLIARSVubiquitination[1]
271FIDCFLTKMAQEKQDacetylation[2]
271FIDCFLTKMAQEKQDubiquitination[1]
319LILMKYPKVQARVQEubiquitination[1]
421LDDNHSFKKSPAFMPubiquitination[1]
422DDNHSFKKSPAFMPFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Involved in the regio- and stereoselective transformation of naphthalene to trans-1R-hydroxy-2R-glutathionyl- 1,2-dihydronaphthalene in the presence of glutathione and glutathione S-transferases. It specifically catalyzes the production of a very reactive and potentially toxic intermediate, the 2R,2S arene oxide, that is associated with necrosis of the unciliated bronchiolar epithelial cells or Clara cells in lung. 
Sequence Annotation
 METAL 436 436 Iron (heme axial ligand) (By similarity).  
Keyword
 Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 491 AA 
Protein Sequence
MDGVSTAILL LLLAVISLSL TFSSRGKGQL PPGPKPLPIL GNLLQLRSQD LLTSLTKLSK 60
EYGSVFTVYL GSRPVIVLSG YQTVKEALVD KGEEFSGRGA YPVFFNFTRG NGIAFSDGER 120
WKILRRFSVQ ILRNFGMGKR SIEERILEEG SFLLEVLRKM EGKPFDPVFI LSRSVSNIIC 180
SVVFGSRFDY DDERLLTIIH FINDNFKIMS SPWGEMYNIF PSVLDWIPGP HKRLFRNFGG 240
MKDLIARSVR EHQDSLDPNS PRDFIDCFLT KMAQEKQDPL SHFNMDTLLM TTHNLLFGGT 300
ETVGTTLRHA FLILMKYPKV QARVQEEIDR VVGRSRMPTL EDRTSMPYTD AVIHEVQRFA 360
DVIPMNLPHR VTRDTPFRGF LIPKGTDVIT LLNTVHYDSD QFKTPQEFNP EHFLDDNHSF 420
KKSPAFMPFS AGRRLCLGEP LARMELFIYF TSILQNFTLQ PLVDPEDIDL TPLSSGLGNL 480
PRPFQLCMHI R 491 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0016712; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; IEA:Compara.
 GO:0019825; F:oxygen binding; IEA:InterPro.
 GO:0018931; P:naphthalene metabolic process; IMP:MGI.
 GO:0009636; P:response to toxic substance; IMP:MGI.
 GO:0018979; P:trichloroethylene metabolic process; IEA:Compara. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR020469; Cyt_P450_CYP2_fam.
 IPR002401; Cyt_P450_E_grp-I. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR01957; EP450ICYP2F.
 PR00385; P450.