CPLM-012628

Genbank Nucleotide ID

Neutral amino acid transporter B(0)

Protein Synonyms/Alias

ATB(0); Baboon M7 virus receptor; RD114/simian type D retrovirus receptor; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5

ASCT2; M7V1; RDR; RDRC

Homo sapiens (Human)

Position	Peptide	Type	References
10	ADPPRDSKGLAAAEP	ubiquitination	[1, 2, 3, 4]
247	VFGVALRKLGPEGEL	ubiquitination	[4, 5]
372	EENNGVAKHISRFIL	ubiquitination	[2, 4, 5, 6, 7]
502	EPELIQVKSELPLDP	ubiquitination	[2, 3, 4, 5, 6, 7, 8, 9, 10, 11]
522	EEGNPLLKHYRGPAG	ubiquitination	[2, 3, 4, 5, 6, 7, 9, 11]
537	DATVASEKESVM***	ubiquitination	[2, 3, 4, 5, 7]

[1] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[11] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses.

MOD_RES 1 1 N-acetylmethionine.
MOD_RES 493 493 Phosphoserine.
MOD_RES 503 503 Phosphoserine.
MOD_RES 535 535 Phosphoserine.
CARBOHYD 163 163 N-linked (GlcNAc...) (Potential).
CARBOHYD 212 212 N-linked (GlcNAc...).

Acetylation; Alternative initiation; Alternative splicing; Complete proteome; Glycoprotein; Host cell receptor for virus entry; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005794; C:Golgi apparatus; IDA:HPA.
GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
GO:0015186; F:L-glutamine transmembrane transporter activity; TAS:BHF-UCL.
GO:0004872; F:receptor activity; TAS:ProtInc.
GO:0017153; F:sodium:dicarboxylate symporter activity; IEA:InterPro.

IPR001991; Na-dicarboxylate_symporter.
IPR018107; Na-dicarboxylate_symporter_CS.

PS00713; NA_DICARBOXYL_SYMP_1
PS00714; NA_DICARBOXYL_SYMP_2

PR00173; EDTRNSPORT.