CPLM-020200

Genbank Nucleotide ID

Programmed cell death protein 10

Protein Synonyms/Alias

Cerebral cavernous malformations 3 protein; TF-1 cell apoptosis-related protein 15

Homo sapiens (Human)

Position	Peptide	Type	References
70	IMKILEKKSVEVNFT	ubiquitination	[1]
106	EFQDLNEKARALKQI	ubiquitination	[2, 3]
132	VRFLQTIKDIASAIK	ubiquitination	[4, 5, 6]
150	DTVNNVFKKYQYQNR	ubiquitination	[4, 6]
179	KSFSDTLKTYFKDGK	acetylation	[7]
179	KSFSDTLKTYFKDGK	ubiquitination	[4, 6]
183	DTLKTYFKDGKAINV	ubiquitination	[8]

[1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).

MOD_RES 179 179 N6-acetyllysine.

3D-structure; Acetylation; Angiogenesis; Apoptosis; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Golgi apparatus; Membrane; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:UniProtKB.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB.
GO:0043406; P:positive regulation of MAP kinase activity; IDA:UniProtKB.