CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024003
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat and SOCS box-containing protein 1 
Protein Synonyms/Alias
 WSB-1; SOCS box-containing WD protein SWiP-1 
Gene Name
 WSB1 
Gene Synonyms/Alias
 SWIP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11FPPRVNEKEIVRLRTubiquitination[1]
31APAAPFDKKCGRENWubiquitination[2, 3, 4]
32PAAPFDKKCGRENWTubiquitination[1]
59SQGHRTVKLVPWSQCubiquitination[1]
201TLRVWDLKDDGNMMKubiquitination[1]
208KDDGNMMKVLRGHQNubiquitination[1, 2, 4]
333VASLADDKMVRFWRIubiquitination[1, 3, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes type II iodothyronine deiodinase/DIO2. Confers constitutive instability to HIPK2 through proteasomal degradation. 
Sequence Annotation
 REPEAT 32 71 WD 1.
 REPEAT 124 165 WD 2.
 REPEAT 168 208 WD 3.
 REPEAT 212 251 WD 4.
 REPEAT 254 293 WD 5.
 REPEAT 309 346 WD 6.
 DOMAIN 372 421 SOCS box.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MASFPPRVNE KEIVRLRTIG ELLAPAAPFD KKCGRENWTV AFAPDGSYFA WSQGHRTVKL 60
VPWSQCLQNF LLHGTKNVTN SSSLRLPRQN SDGGQKNKPR EHIIDCGDIV WSLAFGSSVP 120
EKQSRCVNIE WHRFRFGQDQ LLLATGLNNG RIKIWDVYTG KLLLNLVDHT EVVRDLTFAP 180
DGSLILVSAS RDKTLRVWDL KDDGNMMKVL RGHQNWVYSC AFSPDSSMLC SVGASKAVFL 240
WNMDKYTMIR KLEGHHHDVV ACDFSPDGAL LATASYDTRV YIWDPHNGDI LMEFGHLFPP 300
PTPIFAGGAN DRWVRSVSFS HDGLHVASLA DDKMVRFWRI DEDYPVQVAP LSNGLCCAFS 360
TDGSVLAAGT HDGSVYFWAT PRQVPSLQHL CRMSIRRVMP TQEVQELPIP SKLLEFLSYR 420
I 421 
Gene Ontology
 GO:0005622; C:intracellular; IDA:LIFEdb.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR001496; SOCS_C.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF07525; SOCS_box
 PF00400; WD40 
SMART
 SM00253; SOCS
 SM00969; SOCS_box
 SM00320; WD40 
PROSITE
 PS50225; SOCS
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.