CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017993
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein CIP2A 
Protein Synonyms/Alias
 Cancerous inhibitor of PP2A; p90 autoantigen 
Gene Name
 KIAA1524 
Gene Synonyms/Alias
 CIP2A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MDSTACLKSLLLTVSubiquitination[1]
18LLTVSQYKAVKSEANubiquitination[1]
21VSQYKAVKSEANATQubiquitination[1]
40LEVISGQKLTRLFTSubiquitination[1, 2, 3]
473VADSELCKLAADVILubiquitination[1]
502GMEVSFYKILQDPRLubiquitination[1]
589KCLTPHLKDGVPGLNubiquitination[1, 4]
611LQSGMVVKDQICDVRubiquitination[1, 4]
629IMDVYEMKLSTLASKubiquitination[1, 2]
636KLSTLASKESRLQDLubiquitination[1]
647LQDLLETKALALAQAubiquitination[1, 2, 3, 4, 5, 6, 7]
685MLREVERKNEELSVLubiquitination[1]
694EELSVLLKAQQVESEubiquitination[1]
716HLFQHNRKLESVAEEubiquitination[1, 3]
746ESTEKKNKDLQITCDubiquitination[1]
820EKIKTLQKEREDKEEacetylation[8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
 Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 904 904 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 905 AA 
Protein Sequence
MDSTACLKSL LLTVSQYKAV KSEANATQLL RHLEVISGQK LTRLFTSNQI LTSECLSCLV 60
ELLEDPNISA SLILSIIGLL SQLAVDIETR DCLQNTYNLN SVLAGVVCRS SHTDSVFLQC 120
IQLLQKLTYN VKIFYSGANI DELITFLIDH IQSSEDELKM PCLGLLANLC RHNLSVQTHI 180
KTLSNVKSFY RTLITLLAHS SLTVVVFALS ILSSLTLNEE VGEKLFHARN IHQTFQLIFN 240
ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL 300
ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV 360
LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI 420
EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL 480
KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD 540
FPALVLGESI AANNAYRQQE TEHIPRKMPW QSSNHSFPTS IKCLTPHLKD GVPGLNIEEL 600
IEKLQSGMVV KDQICDVRIS DIMDVYEMKL STLASKESRL QDLLETKALA LAQADRLIAQ 660
HRCQRTQAET EARTLASMLR EVERKNEELS VLLKAQQVES ERAQSDIEHL FQHNRKLESV 720
AEEHEILTKS YMELLQRNES TEKKNKDLQI TCDSLNKQIE TVKKLNESLK EQNEKSIAQL 780
IEKEEQRKEV QNQLVDREHK LANLHQKTKV QEEKIKTLQK EREDKEETID ILRKELSRTE 840
QIRKELSIKA SSLEVQKAQL EGRLEEKESL VKLQQEELNK HSHMIAMIHS LSGGKINPET 900
VNLSI 905 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:HPA. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold. 
Pfam
  
SMART
  
PROSITE
  
PRINTS