CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023587
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 UPF0568 protein C14orf166 
Protein Synonyms/Alias
 CLE7 homolog; CLE 
Gene Name
 C14orf166 
Gene Synonyms/Alias
 CGI-99 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20NPAGFNCKDETEFRNacetylation[1]
20NPAGFNCKDETEFRNubiquitination[2]
41DQKIRHYKIEDRGNLacetylation[1]
41DQKIRHYKIEDRGNLubiquitination[3]
58IHSSDWPKFFEKYLRacetylation[1]
58IHSSDWPKFFEKYLRubiquitination[2, 3]
62DWPKFFEKYLRDVNCacetylation[1]
72RDVNCPFKIQDRQEAubiquitination[2, 3]
98EYGDNAEKYKDLVPDacetylation[1]
98EYGDNAEKYKDLVPDubiquitination[3]
115KTADNATKNAEPLINubiquitination[2, 4, 5, 6]
154DDYLVMLKAIRILVQubiquitination[2, 3, 4, 6]
171LTQDAVAKANQTKEGubiquitination[2, 4, 5, 6, 7]
176VAKANQTKEGLPVALacetylation[8]
176VAKANQTKEGLPVALubiquitination[2, 3, 6, 9]
185GLPVALDKHILGFDTacetylation[10]
218ELRELQTKINEAIVAubiquitination[2, 11]
234QAIIADPKTDHRLGKubiquitination[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [11] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 Involved in modulation of mRNA transcription by Polymerase II. In case of infection by influenza virus A, is involved in viral replication. 
Sequence Annotation
 MOD_RES 20 20 N6-acetyllysine.
 MOD_RES 62 62 N6-acetyllysine.
 MOD_RES 98 98 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Host-virus interaction; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 244 AA 
Protein Sequence
MFRRKLTALD YHNPAGFNCK DETEFRNFIV WLEDQKIRHY KIEDRGNLRN IHSSDWPKFF 60
EKYLRDVNCP FKIQDRQEAI DWLLGLAVRL EYGDNAEKYK DLVPDNSKTA DNATKNAEPL 120
INLDVNNPDF KAGVMALANL LQIQRHDDYL VMLKAIRILV QERLTQDAVA KANQTKEGLP 180
VALDKHILGF DTGDAVLNEA AQILRLLHIE ELRELQTKIN EAIVAVQAII ADPKTDHRLG 240
KVGR 244 
Gene Ontology
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0072669; C:tRNA-splicing ligase complex; IDA:UniProtKB.
 GO:0000993; F:RNA polymerase II core binding; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR019265; UPF0568. 
Pfam
 PF10036; RLL 
SMART
  
PROSITE
  
PRINTS