UniProt Accession

 MARE1_MOUSE; Q61166; Q7TN34 

Genbank Protein ID

 U51196; BC052405; BC064444 

Genbank Nucleotide ID

 AAA96320.1; AAH52405.1; AAH64444.1 

Protein Name

 Microtubule-associated protein RP/EB family member 1 

Protein Synonyms/Alias

 APC-binding protein EB1; End-binding protein 1; EB1 

Gene Name

 Mapre1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	KKVKFQAKLEHEYIQ	acetylation	[1]
76	HEYIQNFKILQAGFK	acetylation	[1]
83	KILQAGFKRMGVDKI	acetylation	[1]
89	FKRMGVDKIIPVDKL	acetylation	[1]
95	DKIIPVDKLVKGKFQ	acetylation	[2]
122	FDANYDGKEYDPVAA	acetylation	[1]
182	AGPGMVRKNPGVGNG	ubiquitination	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (By similarity). May play a role in cell migration. 

Sequence Annotation

 DOMAIN 14 116 CH.
 DOMAIN 185 255 EB1 C-terminal.
 REGION 124 268 Interaction with MTUS2/TIP150 (By
 REGION 208 268 DCTN1-binding (By similarity).
 REGION 220 242 APC-binding (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 124 124 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Microtubule; Mitosis; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 268 AA 

Protein Sequence

Gene Ontology

 GO:0031253; C:cell projection membrane; IDA:MGI.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0030981; C:cortical microtubule cytoskeleton; ISS:UniProtKB.
 GO:0005881; C:cytoplasmic microtubule; IDA:MGI.
 GO:0005794; C:Golgi apparatus; IDA:MGI.
 GO:0035371; C:microtubule plus end; IDA:MGI.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0051010; F:microtubule plus-end binding; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031115; P:negative regulation of microtubule polymerization; ISS:UniProtKB.
 GO:0035372; P:protein localization to microtubule; ISS:UniProtKB. 

Interpro

 IPR001715; CH-domain.
 IPR004953; EB1_C.
 IPR027328; MAPRE. 

Pfam

 PF00307; CH
 PF03271; EB1 

SMART

  

PROSITE

 PS50021; CH
 PS51230; EB1_C 

PRINTS