UniProt Accession

 PDK2_MOUSE; Q9JK42; Q8VC63 

Genbank Protein ID

 AF267660; AL606480; CH466556; BC021764 

Genbank Nucleotide ID

 AAF72038.1; CAI23968.1; EDL15963.1; AAH21764.1 

Protein Name

 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial 

Protein Synonyms/Alias

 Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2 

Gene Name

 Pdk2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
9	RWVRALLKNASLAGA	ubiquitination	[1]
25	KYIEHFSKFSPSPLS	ubiquitination	[1]
376	ERLPVYNKSAWRHYQ	acetylation	[2]
376	ERLPVYNKSAWRHYQ	succinylation	[2]
376	ERLPVYNKSAWRHYQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Serine/threonine kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. 

Sequence Annotation

 DOMAIN 135 364 Histidine kinase.
 NP_BIND 251 258 ATP (By similarity).
 NP_BIND 309 310 ATP (By similarity).
 NP_BIND 325 330 ATP (By similarity).
 BINDING 290 290 ATP (By similarity).  

Keyword

 ATP-binding; Carbohydrate metabolism; Complete proteome; Glucose metabolism; Kinase; Mitochondrion; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 407 AA 

Protein Sequence

Gene Ontology

 GO:0005967; C:mitochondrial pyruvate dehydrogenase complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004740; F:pyruvate dehydrogenase (acetyl-transferring) kinase activity; IMP:UniProtKB.
 GO:0031670; P:cellular response to nutrient; IMP:UniProtKB.
 GO:0034614; P:cellular response to reactive oxygen species; IEA:Compara.
 GO:0042593; P:glucose homeostasis; IMP:UniProtKB.
 GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; IMP:UniProtKB.
 GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; IEA:Compara.
 GO:0010510; P:regulation of acetyl-CoA biosynthetic process from pyruvate; IMP:UniProtKB.
 GO:0006111; P:regulation of gluconeogenesis; IMP:UniProtKB.
 GO:0006885; P:regulation of pH; IMP:UniProtKB. 

Interpro

 IPR018955; BCDHK/PDK_N.
 IPR003594; HATPase_ATP-bd.
 IPR005467; Sig_transdc_His_kinase_core. 

Pfam

 PF10436; BCDHK_Adom3
 PF02518; HATPase_c 

SMART

 SM00387; HATPase_c 

PROSITE

 PS50109; HIS_KIN 

PRINTS