CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023978
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein O-mannosyl-transferase 1 
Protein Synonyms/Alias
 Dolichyl-phosphate-mannose--protein mannosyltransferase 1 
Gene Name
 POMT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
335TLRNVFGKPVPCWLHubiquitination[1]
370QVTCYPFKDVNNWWIubiquitination[1]
379VNNWWIVKDPRRHQLubiquitination[1, 2]
459GSDTDVWKTILSEVRubiquitination[1, 2, 3]
497QLEIVGEKLSRGYHGubiquitination[1, 2]
732SLSPHELKALRWKDSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. 
Sequence Annotation
 DOMAIN 318 381 MIR 1.
 DOMAIN 392 449 MIR 2.
 DOMAIN 453 513 MIR 3.
 CARBOHYD 435 435 N-linked (GlcNAc...) (Potential).
 CARBOHYD 471 471 N-linked (GlcNAc...).
 CARBOHYD 539 539 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Congenital muscular dystrophy; Disease mutation; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Limb-girdle muscular dystrophy; Lissencephaly; Membrane; Metal-binding; Polymorphism; Reference proteome; Repeat; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MWGFLKRPVV VTADINLSLV ALTGMGLLSR LWRLTYPRAV VFDEVYYGQY ISFYMKQIFF 60
LDDSGPPFGH MVLALGGYLG GFDGNFLWNR IGAEYSSNVP VWSLRLLPAL AGALSVPMAY 120
QIVLELHFSH CAAMGAALLM LIENALITQS RLMLLESVLI FFNLLAVLSY LKFFNCQKHS 180
PFSLSWWFWL TLTGVACSCA VGIKYMGVFT YVLVLGVAAV HAWHLLGDQT LSNVGADVQC 240
CMRPACMGQM QMSQGVCVFC HLLARAVALL VIPVVLYLLF FYVHLILVFR SGPHDQIMSS 300
AFQASLEGGL ARITQGQPLE VAFGSQVTLR NVFGKPVPCW LHSHQDTYPM IYENGRGSSH 360
QQQVTCYPFK DVNNWWIVKD PRRHQLVVSS PPRPVRHGDM VQLVHGMTTR SLNTHDVAAP 420
LSPHSQEVSC YIDYNISMPA QNLWRLEIVN RGSDTDVWKT ILSEVRFVHV NTSAVLKLSG 480
AHLPDWGYRQ LEIVGEKLSR GYHGSTVWNV EEHRYGASQE QRERERELHS PAQVDVSRNL 540
SFMARFSELQ WRMLALRSDD SEHKYSSSPL EWVTLDTNIA YWLHPRTSAQ IHLLGNIVIW 600
VSGSLALAIY ALLSLWYLLR RRRNVHDLPQ DAWLRWVLAG ALCAGGWAVN YLPFFLMEKT 660
LFLYHYLPAL TFQILLLPVV LQHISDHLCR SQLQRSIFSA LVVAWYSSAC HVSNTLRPLT 720
YGDKSLSPHE LKALRWKDSW DILIRKH 747 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0016529; C:sarcoplasmic reticulum; IEA:Compara.
 GO:0004169; F:dolichyl-phosphate-mannose-protein mannosyltransferase activity; IEA:EC.
 GO:0000030; F:mannosyltransferase activity; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030198; P:extracellular matrix organization; IEA:Compara.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0006493; P:protein O-linked glycosylation; TAS:ProtInc. 
Interpro
 IPR027005; GlyclTrfase_39_like.
 IPR003342; Glyco_trans_39.
 IPR016093; MIR_motif. 
Pfam
 PF02815; MIR
 PF02366; PMT 
SMART
 SM00472; MIR 
PROSITE
 PS50919; MIR 
PRINTS