CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras GTPase-activating protein 2 
Protein Synonyms/Alias
 GTPase-activating protein 1m; GAP1m 
Gene Name
 RASA2 
Gene Synonyms/Alias
 GAP1M; RASGAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
134DLCNHSGKETWFSLQubiquitination[1]
208GPSRNDQKKTKVKKKacetylation[2]
209PSRNDQKKTKVKKKTacetylation[2]
211RNDQKKTKVKKKTSNacetylation[2]
213DQKKTKVKKKTSNPQacetylation[2]
302DNGNKSSKTDDLGSLubiquitination[1]
357LSEICRDKNDAVLPLubiquitination[1]
419IVGGHYLKVTLKPILubiquitination[1]
462NLRYYVDKLFNTIVKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4). 
Sequence Annotation
 DOMAIN 25 122 C2 1.
 DOMAIN 158 273 C2 2.
 DOMAIN 356 550 Ras-GAP.
 DOMAIN 604 706 PH.
 ZN_FING 708 744 Btk-type.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; GTPase activation; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 850 AA 
Protein Sequence
MAAAAPAAAA ASSEAPAASA TAEPEAGDQD SREVRVLQSL RGKICEAKNL LPYLGPHKMR 60
DCFCTINLDQ EEVYRTQVVE KSLSPFFSEE FYFEIPRTFQ YLSFYVYDKN VLQRDLRIGK 120
VAIKKEDLCN HSGKETWFSL QPVDSNSEVQ GKVHLELKLN ELITENGTVC QQLVVHIKAC 180
HGLPLINGQS CDPYATVSLV GPSRNDQKKT KVKKKTSNPQ FNEIFYFEVT RSSSYTRKSQ 240
FQVEEEDIEK LEIRIDLWNN GNLVQDVFLG EIKVPVNVLR TDSSHQAWYL LQPRDNGNKS 300
SKTDDLGSLR LNICYTEDYV LPSEYYGPLK TLLLKSPDVQ PISASAAYIL SEICRDKNDA 360
VLPLVRLLLH HDKLVPFATA VAELDLKDTQ DANTIFRGNS LATRCLDEMM KIVGGHYLKV 420
TLKPILDEIC DSSKSCEIDP IKLKEGDNVE NNKENLRYYV DKLFNTIVKS SMSCPTVMCD 480
IFYSLRQMAT QRFPNDPHVQ YSAVSSFVFL RFFAVAVVSP HTFHLRPHHP DAQTIRTLTL 540
ISKTIQTLGS WGSLSKSKSS FKETFMCEFF KMFQEEGYII AVKKFLDEIS STETKESSGT 600
SEPVHLKEGE MYKRAQGRTR IGKKNFKKRW FCLTSRELTY HKQPGSKDAI YTIPVKNILA 660
VEKLEESSFN KKNMFQVIHT EKPLYVQANN CVEANEWIDV LCRVSRCNQN RLSFYHPSVY 720
LNGNWLCCQE TGENTLGCKP CTAGVPADIQ IDIDEDRETE RIYSLFTLSL LKLQKMEEAC 780
GTIAVYQGPQ KEPDDYSNFV IEDSVTTFKT IQQIKSIIEK LDEPHEKYRK KRSSSAKYGS 840
KENPIVGKAS 850 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IBA:RefGenome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005099; F:Ras GTPase activator activity; IBA:RefGenome.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:RefGenome.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001562; Znf_Btk_motif. 
Pfam
 PF00779; BTK
 PF00168; C2
 PF00169; PH
 PF00616; RasGAP 
SMART
 SM00107; BTK
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2
 PS51113; ZF_BTK 
PRINTS
 PR00402; TECBTKDOMAIN.