CPLM-006427

Genbank Nucleotide ID

Transcription initiation factor TFIID subunit 14

Protein Synonyms/Alias

Actin non-complementing mutant 1; Chromosome stability protein 10; SWI/SNF chromatin-remodeling complex subunit TAF14; SWI/SNF complex 29 kDa subunit; SWI/SNF complex subunit TAF14; TBP-associated factor 14; TBP-associated factor 30 kDa; Transcription factor G 30 kDa subunit; Transcription initiation factor TFIIF 30 kDa subunit

ANC1; CST10; SWP29; TAF30; TFG3; YPL129W

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
149	ANTGTIGKRRTTTNT	ubiquitination	[1]
181	KGSVDLEKLAFGLTK	acetylation	[2]
181	KGSVDLEKLAFGLTK	ubiquitination	[1, 3]

[1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
[3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]

Functional Description

Functions as a component of the DNA-binding general transcription factor complex TFIID, the RNA polymerase II associated general transcription factor complex TFIIF, and the chromatin-remodeling complex SWI/SNF. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TFIIF is essential for the initiation of transcription by RNA polymerase II. TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. The TAF14 subunit has stimulatory activity. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. Component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation.

3D-structure; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031011; C:Ino80 complex; IDA:SGD.
GO:0016592; C:mediator complex; IPI:SGD.
GO:0033100; C:NuA3 histone acetyltransferase complex; IDA:SGD.
GO:0016586; C:RSC complex; IPI:SGD.
GO:0016514; C:SWI/SNF complex; IDA:SGD.
GO:0005669; C:transcription factor TFIID complex; IDA:SGD.
GO:0005674; C:transcription factor TFIIF complex; IDA:SGD.
GO:0000991; F:core RNA polymerase II binding transcription factor activity; IDA:SGD.
GO:0016573; P:histone acetylation; IDA:SGD.
GO:0042766; P:nucleosome mobilization; IDA:SGD.
GO:0001111; P:promoter clearance from RNA polymerase II promoter; IC:SGD.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IC:SGD.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; IC:SGD.
GO:0001174; P:transcriptional start site selection at RNA polymerase II promoter; IC:SGD.

IPR016665; SAS_cplx_Sas5/TFIID_TAF14.
IPR005033; YEATS.