UniProt Accession

 EM55_HUMAN; Q00013; B4DZV5; G3XAI1; Q2TSB6; Q5J7V5 

Genbank Protein ID

 M64925; M87059; U39611; AY423731; AK290246; AK303111; AK312296; AK315957; AY634686; AC109993; CH471172; CH471172; CH471172; BC002392 

Genbank Nucleotide ID

 AAA60059.1; AAA60060.1; AAD14835.1; AAS00494.1; BAF82935.1; BAG64217.1; BAG35223.1; BAH14328.1; AAV35469.1; EAW72655.1; EAW72656.1; EAW72660.1; AAH02392.1 

Protein Name

 55 kDa erythrocyte membrane protein 

Protein Synonyms/Alias

 p55; Membrane protein, palmitoylated 1 

Gene Name

 MPP1 

Gene Synonyms/Alias

 DXS552E; EMP55 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
91	ITLKLNEKQSCTVAR	ubiquitination	[1]
147	TKGMISLKVIPNQQS	ubiquitination	[1]
180	KDNLIPCKEAGLKFA	ubiquitination	[1]
197	DIIQIINKDDSNWWQ	ubiquitination	[1]
212	GRVEGSSKESAGLIP	ubiquitination	[1]
247	PSCSPFGKKKKYKDK	ubiquitination	[1, 2]
248	SCSPFGKKKKYKDKY	ubiquitination	[1]
299	GVGRSHIKNALLSQN	ubiquitination	[1]
323	YTTRPPRKSEEDGKE	ubiquitination	[1]
329	RKSEEDGKEYHFIST	ubiquitination	[1]
371	ETVHQIHKQNKIAIL	ubiquitination	[3]
374	HQIHKQNKIAILDIE	ubiquitination	[3]
386	DIEPQTLKIVRTAEL	ubiquitination	[1, 3]
417	EALQQLQKDSEAIRS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity). 

Sequence Annotation

 DOMAIN 71 152 PDZ.
 DOMAIN 158 228 SH3.
 DOMAIN 282 451 Guanylate kinase-like.
 REGION 268 466 Interaction with MPP5.
 MOD_RES 19 19 Phosphoserine (By similarity).
 MOD_RES 49 49 Phosphothreonine.
 MOD_RES 57 57 Phosphoserine.
 MOD_RES 110 110 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Cell projection; Complete proteome; Direct protein sequencing; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 466 AA 

Protein Sequence

Gene Ontology

 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005622; C:intracellular; IDA:LIFEdb.
 GO:0032420; C:stereocilium; IEA:UniProtKB-SubCell.
 GO:0004385; F:guanylate kinase activity; TAS:ProtInc.
 GO:0090022; P:regulation of neutrophil chemotaxis; ISS:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

 IPR008144; Guanylate_kin.
 IPR008145; Guanylate_kin/L-typ_Ca_channel.
 IPR020590; Guanylate_kinase_CS.
 IPR027417; P-loop_NTPase.
 IPR001478; PDZ.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 

Pfam

 PF00625; Guanylate_kin
 PF00595; PDZ
 PF07653; SH3_2 

SMART

 SM00072; GuKc
 SM00228; PDZ
 SM00326; SH3 

PROSITE

 PS00856; GUANYLATE_KINASE_1
 PS50052; GUANYLATE_KINASE_2
 PS50106; PDZ
 PS50002; SH3 

PRINTS