UniProt Accession

 ODO2_HUMAN; P36957; Q7LDY7; Q9BQ32 

Genbank Protein ID

 D16373; D26535; L37418; AK289414; AC006530; CH471061; BC000302; BC001922 

Genbank Nucleotide ID

 BAA03871.1; BAA05536.1; AAB59629.1; BAF82103.1; AAD30181.1; EAW81199.1; AAH00302.1; AAH01922.1 

Protein Name

 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial 

Protein Synonyms/Alias

 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; E2K 

Gene Name

 DLST 

Gene Synonyms/Alias

 DLTS 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
267	QEMRARHKEAFLKKH	acetylation	[1]
272	RHKEAFLKKHNLKLG	acetylation	[1]
277	FLKKHNLKLGFMSAF	ubiquitination	[2]
353	TITELGEKARKNELA	ubiquitination	[3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). 

Sequence Annotation

 DOMAIN 71 143 Lipoyl-binding.
 ACT_SITE 424 424 Potential.
 ACT_SITE 428 428 Potential.
 MOD_RES 110 110 N6-lipoyllysine (Potential).  

Keyword

 Acyltransferase; Complete proteome; Direct protein sequencing; Lipoyl; Mitochondrion; Polymorphism; Reference proteome; Transferase; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 453 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0045252; C:oxoglutarate dehydrogenase complex; IEA:InterPro.
 GO:0004149; F:dihydrolipoyllysine-residue succinyltransferase activity; IEA:EC.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0033512; P:L-lysine catabolic process to acetyl-CoA via saccharopine; IEA:UniProtKB-UniPathway.
 GO:0006554; P:lysine catabolic process; TAS:Reactome.
 GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome. 

Interpro

 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR011053; Single_hybrid_motif.
 IPR006255; SucB. 

Pfam

 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl 

SMART

  

PROSITE

 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 

PRINTS