CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023642
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Epididymis-specific alpha-mannosidase 
Protein Synonyms/Alias
 Mannosidase alpha class 2B member 2 
Gene Name
 MAN2B2 
Gene Synonyms/Alias
 KIAA0935 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
93GVASDQQKYQVRQLLubiquitination[1, 2]
181SRIDYDLKAAMQEARubiquitination[1, 2]
768SAFMEDGKSRLVLLSubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
 ACT_SITE 151 151 Nucleophile (By similarity).
 METAL 36 36 Zinc (By similarity).
 METAL 38 38 Zinc (By similarity).
 METAL 151 151 Zinc (By similarity).
 METAL 420 420 Zinc (By similarity).
 CARBOHYD 226 226 N-linked (GlcNAc...) (Potential).
 CARBOHYD 249 249 N-linked (GlcNAc...) (Potential).
 CARBOHYD 294 294 N-linked (GlcNAc...) (Potential).
 CARBOHYD 336 336 N-linked (GlcNAc...) (Potential).
 CARBOHYD 516 516 N-linked (GlcNAc...).
 CARBOHYD 608 608 N-linked (GlcNAc...) (Potential).
 CARBOHYD 670 670 N-linked (GlcNAc...) (Potential).
 CARBOHYD 675 675 N-linked (GlcNAc...) (Potential).
 CARBOHYD 748 748 N-linked (GlcNAc...) (Potential).
 CARBOHYD 808 808 N-linked (GlcNAc...) (Potential).
 CARBOHYD 812 812 N-linked (GlcNAc...) (Potential).
 CARBOHYD 890 890 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Glycosidase; Hydrolase; Metal-binding; Polymorphism; Reference proteome; Secreted; Signal; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1009 AA 
Protein Sequence
MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR AYAANVYTSV 60
VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL EEGRLEFVIG GQVMHDEAVT 120
HLDDQILQLT EGHGFLYETF GIRPQFSWHV DPFGASATTP TLFALAGFNA HLGSRIDYDL 180
KAAMQEARGL QFVWRGSPSL SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK 240
PPQDGVYPNM SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA 300
NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY STEPFQAWTG 360
FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP TWALQQLQQL RWAVSEVQHH 420
DAITGTESPK VRDMYATHLA SGMLGMRKLM ASIVLDELQP QAPMAASSDA GPAGHFASVY 480
NPLAWTVTTI VTLTVGFPGV RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY 540
NIRPTAGAQE GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH 600
SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV EMEIVAGQLV 660
TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI EQEYQAGPLE LNREAVLRTS 720
TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS IARNYYPMVQ SAFMEDGKSR LVLLSERAHG 780
ISSQGNGQVE VMLHRRLWNN FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL 840
ALQHRPVVLF GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK 900
GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV EERSLTGTWD 960
LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR TFFIHFQQQ 1009 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IEA:Compara.
 GO:0004559; F:alpha-mannosidase activity; IEA:EC.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006013; P:mannose metabolic process; IEA:Compara. 
Interpro
 IPR011013; Gal_mutarotase_SF_dom.
 IPR011330; Glyco_hydro/deAcase_b/a-brl.
 IPR013780; Glyco_hydro_13_b.
 IPR027291; Glyco_hydro_38/57_N.
 IPR011682; Glyco_hydro_38_C.
 IPR015341; Glyco_hydro_38_cen_dom.
 IPR000602; Glyco_hydro_38_N. 
Pfam
 PF09261; Alpha-mann_mid
 PF01074; Glyco_hydro_38
 PF07748; Glyco_hydro_38C 
SMART
 SM00872; Alpha-mann_mid 
PROSITE
  
PRINTS