UniProt Accession

 IMDH2_YEAST; P38697; D3DLH1 

Genbank Protein ID

 U00029; BK006934 

Genbank Nucleotide ID

 AAB69728.1; DAA06915.1 

Protein Name

 Inosine-5'-monophosphate dehydrogenase 2 

Protein Synonyms/Alias

 IMP dehydrogenase 2; IMPD 2; IMPDH 2 

Gene Name

 IMD2 

Gene Synonyms/Alias

 PUR5; YHR216W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
143	EAKSMKEKYGFAGFP	acetylation	[1]
205	SEGNEILKKIKKGRL	acetylation	[1]
205	SEGNEILKKIKKGRL	ubiquitination	[2]
519	HNLHSYEKRLHN***	acetylation	[1]
519	HNLHSYEKRLHN***	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. In contrast to the other IMPDH alleles IMD3 and IMD4, the enzymatic activity of IMD2 seems to be intrinsically drug resistant. 

Sequence Annotation

 DOMAIN 121 183 CBS 1.
 DOMAIN 184 240 CBS 2.
 NP_BIND 278 280 NAD (By similarity).
 NP_BIND 328 330 NAD (By similarity).
 REGION 368 370 IMP binding (By similarity).
 REGION 391 392 IMP binding (By similarity).
 REGION 415 419 IMP binding (By similarity).
 ACT_SITE 335 335 Thioimidate intermediate (By similarity).
 METAL 330 330 Potassium; via carbonyl oxygen (By
 METAL 332 332 Potassium; via carbonyl oxygen (By
 METAL 335 335 Potassium; via carbonyl oxygen (By
 METAL 508 508 Potassium; via carbonyl oxygen; shared
 METAL 509 509 Potassium; via carbonyl oxygen; shared
 METAL 510 510 Potassium; via carbonyl oxygen; shared
 BINDING 333 333 IMP (By similarity).
 BINDING 449 449 IMP (By similarity).  

Keyword

 CBS domain; Complete proteome; Cytoplasm; GMP biosynthesis; Metal-binding; NAD; Oxidoreductase; Potassium; Purine biosynthesis; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 523 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0003938; F:IMP dehydrogenase activity; TAS:SGD.
 GO:0046872; F:metal ion binding; IEA:HAMAP.
 GO:0000166; F:nucleotide binding; IEA:HAMAP.
 GO:0006177; P:GMP biosynthetic process; IEA:HAMAP.
 GO:0006183; P:GTP biosynthetic process; TAS:SGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR000644; Cysta_beta_synth_core.
 IPR005990; IMP_DH.
 IPR015875; IMP_DH/GMP_Rdtase_CS.
 IPR001093; IMP_DH_GMPRt. 

Pfam

 PF00571; CBS
 PF00478; IMPDH 

SMART

 SM00116; CBS 

PROSITE

 PS51371; CBS
 PS00487; IMP_DH_GMP_RED 

PRINTS