CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022055
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 3 
Protein Synonyms/Alias
 Exchange factor found in platelets and leukemic and neuronal tissues; XPLN 
Gene Name
 ARHGEF3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
281LESPFSRKLDLWNFLubiquitination[1, 2]
297IPRSRLVKYPLLLREubiquitination[2]
374LCCHGELKNNRGVKLubiquitination[2]
414YRQPIPVKDLLLEDLubiquitination[2]
443FSNNERIKNFFRVSFubiquitination[2]
451NFFRVSFKNGSQSQTubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases. 
Sequence Annotation
 DOMAIN 122 304 DH.
 DOMAIN 291 449 PH.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 526 AA 
Protein Sequence
MDSSTAMNQC SCRGMEENKE RPKRQRQNNF PMFPSPKAWN FRGRKRKQST QDEDAVSLCS 60
LDISEPSNKR VKPLSRVTSL ANLIPPVKAT PLKRFSQTLQ RSISFRSESR PDILAPRPWS 120
RNAAPSSTKR RDSKLWSETF DVCVNQMLTS KEIKRQEAIF ELSQGEEDLI EDLKLAKKAY 180
HDPMLKLSIM TEQELNQIFG TLDSLIPLHE ELLSQLRDVR KPDGSTEHVG PILVGWLPCL 240
SSYDSYCSNQ VAAKALLDHK KQDHRVQDFL QRCLESPFSR KLDLWNFLDI PRSRLVKYPL 300
LLREILRHTP NDNPDQQHLE EAINIIQGIV AEINTKTGES ECRYYKERLL YLEEGQKDSL 360
IDSSRVLCCH GELKNNRGVK LHVFLFQEVL VITRAVTHNE QLCYQLYRQP IPVKDLLLED 420
LQDGEVRLGG SLRGAFSNNE RIKNFFRVSF KNGSQSQTHS LQANDTFNKQ QWLNCIRQAK 480
ETVLCAAGQA GVLDSEGSFL NPTTGSRELQ GETKLEQMDQ SDSESDCSMD TSEVSLDCER 540
MEQTDSSCGN SRHGESNV 558 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015721; RhoGEF-like. 
Pfam
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS