UniProt Accession

 TRM7_YEAST; P38238; D6VQ61 

Genbank Protein ID

 Z35930; BK006936 

Genbank Nucleotide ID

 CAA85004.1; DAA07181.1 

Protein Name

 tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase 

Protein Synonyms/Alias

 2'-O-ribose RNA methyltransferase TRM7; tRNA methylase 7 

Gene Name

 TRM7 

Gene Synonyms/Alias

 YBR061C; YBR0527 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
111	KTLARILKLFGNEKA	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of tRNA(Phe), tRNA(Trp) and tRNA(Leu(UAA)). Requires TRM732 for methylation of the cytidine at position 32 and RTT10/TRM734 for methylation of the nucleotides at position 34 in substrate tRNAs. Lack of either of these modifications in tRNA(Phe) reduces formation of wybutosine from 1- methylguanosine at position 37. 

Sequence Annotation

 ACT_SITE 164 164 Proton acceptor (By similarity).
 BINDING 55 55 S-adenosyl-L-methionine; via amide
 BINDING 57 57 S-adenosyl-L-methionine; via amide
 BINDING 83 83 S-adenosyl-L-methionine (By similarity).
 BINDING 99 99 S-adenosyl-L-methionine (By similarity).
 BINDING 124 124 S-adenosyl-L-methionine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 310 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0008175; F:tRNA methyltransferase activity; IDA:SGD.
 GO:0002181; P:cytoplasmic translation; IMP:SGD. 

Interpro

 IPR015507; rRNA-MeTfrase_E.
 IPR002877; rRNA_MeTrfase_FtsJ_dom. 

Pfam

 PF01728; FtsJ 

SMART

  

PROSITE

  

PRINTS