CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022342
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/LRR-repeat protein 12 
Protein Synonyms/Alias
 F-box and leucine-rich repeat protein 12; F-box protein FBL12 
Gene Name
 FBXL12 
Gene Synonyms/Alias
 FBL12 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
100LLRALGQKCPNLKRLubiquitination[1]
301WEGQEAEKILCKGLPubiquitination[1]
305EAEKILCKGLPHCMVubiquitination[1, 2, 3]
319VIVRACPKESMDWWMubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity). 
Sequence Annotation
 DOMAIN 1 47 F-box.
 REPEAT 51 78 LRR 1.
 REPEAT 86 111 LRR 2.
 REPEAT 113 133 LRR 3.
 REPEAT 161 185 LRR 4.
 REPEAT 186 211 LRR 5.
 REPEAT 212 236 LRR 6.
 REPEAT 237 261 LRR 7.
 REPEAT 266 291 LRR 8.  
Keyword
 Alternative splicing; Complete proteome; Leucine-rich repeat; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 326 AA 
Protein Sequence
MATLVELPDS VLLEIFSYLP VRDRIRISRV CHRWKRLVDD RWLWRHVDLT LYTMRPKVMW 60
HLLRRYMASR LHSLRMGGYL FSGSQAPQLS PALLRALGQK CPNLKRLCLH VADLSMVPIT 120
SLPSTLRTLE LHSCEISMAW LHKQQDPTVL PLLECIVLDR VPAFRDEHLQ GLTRFRALRS 180
LVLGGTYRVT ETGLDAGLQE LSYLQRLEVL GCTLSADSTL LAISRHLRDV RKIRLTVRGL 240
SAPGLAVLEG MPALESLCLQ GPLVTPEMPS PTEILSSCLT MPKLRVLELQ GLGWEGQEAE 300
KILCKGLPHC MVIVRACPKE SMDWWM 326 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000151; C:ubiquitin ligase complex; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Compara. 
Interpro
 IPR001810; F-box_dom_cyclin-like. 
Pfam
  
SMART
 SM00256; FBOX 
PROSITE
 PS50181; FBOX 
PRINTS