UniProt Accession

 GLPX_ECOLI; P0A9C9; P11007; P28860; P28900; Q2M8M1 

Genbank Protein ID

 Z11767; L19201; U00096; AP009048; M19644 

Genbank Nucleotide ID

 CAA77814.1; AAB03057.1; AAC76907.1; BAE77385.1 

Protein Name

 Fructose-1,6-bisphosphatase 1 class 2 

Protein Synonyms/Alias

 FBPase 1 class 2; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1 class 2 

Gene Name

 glpX 

Gene Synonyms/Alias

 b3925; JW3896 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
29	KWLGRGDKNTADGAA	acetylation	[1]
164	LTVTILAKPRHDAVI	acetylation	[1]
239	LLARHDVKGDNEENR	acetylation	[1, 2]
265	AMGIEAGKVLRLGDM	acetylation	[1]
327	SIHYLDRKDPEMQVH	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. Is likely to be involved in gluconeogenesis during growth on glycerol. Also displays a low activity toward glucose 1,6-bisphosphate, and no activity against ribulose 1,5- bisphosphate, fructose 2,6-bisphosphate, or fructose 1-phosphate. 

Sequence Annotation

 REGION 88 90 Substrate binding.
 REGION 164 166 Substrate binding.
 REGION 186 188 Substrate binding.
 METAL 33 33 Manganese 1.
 METAL 57 57 Manganese 1.
 METAL 85 85 Manganese 2.
 METAL 88 88 Manganese 2.
 METAL 213 213 Manganese 2.
 BINDING 119 119 Substrate.
 BINDING 210 210 Substrate; via amide nitrogen.  

Keyword

 3D-structure; Carbohydrate metabolism; Complete proteome; Cytoplasm; Hydrolase; Manganese; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 336 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0042132; F:fructose 1,6-bisphosphate 1-phosphatase activity; IDA:EcoCyc.
 GO:0030145; F:manganese ion binding; IDA:EcoCyc.
 GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
 GO:0006071; P:glycerol metabolic process; IEA:InterPro. 

Interpro

 IPR004464; FBPase_class-2/SBPase. 

Pfam

 PF03320; FBPase_glpX 

SMART

  

PROSITE

  

PRINTS