UniProt Accession

 GLDA_ECOLI; P0A9S5; P32665; P78132; Q2M8P1 

Genbank Protein ID

 U00006; U00096; AP009048 

Genbank Nucleotide ID

 AAC43051.1; AAC76927.2; BAE77365.1 

Protein Name

 Glycerol dehydrogenase 

Protein Synonyms/Alias

 GDH; GLDH 

Gene Name

 gldA 

Gene Synonyms/Alias

 b3945; JW5556 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
26	NRLGEYLKPLAERWL	acetylation	[1]
50	FAQSTVEKSFKDAGL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. Possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively. 

Sequence Annotation

 NP_BIND 94 98 NAD (By similarity).
 NP_BIND 116 119 NAD (By similarity).
 METAL 171 171 Zinc; catalytic (By similarity).
 METAL 254 254 Zinc; catalytic (By similarity).
 METAL 271 271 Zinc; catalytic (By similarity).
 BINDING 37 37 NAD (By similarity).
 BINDING 121 121 Substrate (By similarity).
 BINDING 125 125 NAD (By similarity).
 BINDING 127 127 NAD; via carbonyl oxygen (By similarity).
 BINDING 131 131 NAD (By similarity).
 BINDING 171 171 Substrate (By similarity).
 BINDING 254 254 Substrate (By similarity).
 BINDING 271 271 Substrate (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; Glycerol metabolism; Metal-binding; NAD; Oxidoreductase; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0019147; F:(R)-aminopropanol dehydrogenase activity; IDA:EcoCyc.
 GO:0008888; F:glycerol dehydrogenase [NAD+] activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019588; P:anaerobic glycerol catabolic process; IMP:EcoCyc.
 GO:0051596; P:methylglyoxal catabolic process; IMP:EcoCyc. 

Interpro

 IPR001670; ADH_Fe.
 IPR018211; ADH_Fe_CS.
 IPR016205; Glycerol_DH. 

Pfam

 PF00465; Fe-ADH 

SMART

  

PROSITE

 PS00913; ADH_IRON_1
 PS00060; ADH_IRON_2 

PRINTS