CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032456
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-activating enzyme E1, Chr X 
Protein Synonyms/Alias
 Ubiquitin-like modifier activating enzyme 1 
Gene Name
 Uba1 
Gene Synonyms/Alias
 Ube1x; mCG_126518 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
185FCHSRGIKLVVADTRubiquitination[1]
296GGIVSQVKVPKKISFubiquitination[1]
299VSQVKVPKKISFKSLubiquitination[1]
304VPKKISFKSLPASLVubiquitination[1]
322FVMTDFAKYSRPAQLubiquitination[1]
374ARSPPSVKQNSLDEDubiquitination[1]
443KEALTEEKCLPRQNRubiquitination[1]
465FGSDFQEKLSKQKYFubiquitination[1]
526FRPWDVTKLKSDTAAubiquitination[1]
528PWDVTKLKSDTAAAAubiquitination[1]
593RRCVYYRKPLLESGTubiquitination[1]
604ESGTLGTKGNVQVVIubiquitination[1]
627SSQDPPEKSIPICTLubiquitination[1]
635SIPICTLKNFPNAIEubiquitination[1]
657DEFEGLFKQPAENVNubiquitination[1]
671NQYLTDSKFVERTLRubiquitination[1]
746APFWSGPKRCPHPLTubiquitination[1]
802QVPEFTPKSGVKIHVubiquitination[1]
806FTPKSGVKIHVSDQEubiquitination[1]
838ATLPSPDKLPGFKMYubiquitination[1]
843PDKLPGFKMYPIDFEubiquitination[1]
851MYPIDFEKDDDSNFHubiquitination[1]
882ISPADRHKSKLIAGKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Ligase; Nucleotide-binding; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1058 AA 
Protein Sequence
MSSSPLSKKR RVSGPDPKPG SNCSPAQSAL SEVSSVPTNG MAKNGSEADI DESLYSRQLY 60
VLGHEAMKML QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTTQWA DLSSQFYLRE 120
EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSSFQ VVVLTNSPLE AQLRVGEFCH 180
SRGIKLVVAD TRGLFGQLFC DFGEEMVLTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH 240
GFETGDFVSF SEVQGMIQLN GCQPMEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK 300
ISFKSLPASL VEPDFVMTDF AKYSRPAQLH IGFQALHQFC ALHNQPPRPR NEEDATELVG 360
LAQAVNARSP PSVKQNSLDE DLIRKLAYVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI 420
MQWLYFDALE CLPEDKEALT EEKCLPRQNR YDGQVAVFGS DFQEKLSKQK YFLVGAGAIG 480
CELLKNFAMI GLGCGEGGEV VVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN 540
PYIQVTSHQN RVGPDTERIY DDDFFQNLDG VANALDNIDA RMYMDRRCVY YRKPLLESGT 600
LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA 660
ENVNQYLTDS KFVERTLRLA GTQPLEVLEA VQRSLVLQRP QTWGDCVTWA CHHWHTQYCN 720
NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVNNTL HLDYVMAAAN LFAQTYGLTG 780
SQDRAAVASL LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP 840
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDISPADR HKSKLIAGKI IPAIATTTAA 900
VVGLVCLELY KVVQGHQQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV 960
QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS 1020
RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR 1058 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008641; F:small protein activating enzyme activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro. 
Interpro
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR018965; Ub-activating_enz_e1_C.
 IPR023280; Ub-like_act_enz_cat_cys_dom.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018075; UBQ-activ_enz_E1.
 IPR018074; UBQ-activ_enz_E1_AS.
 IPR000011; UBQ/SUMO-activ_enz_E1-like. 
Pfam
 PF00899; ThiF
 PF09358; UBA_e1_C
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 
SMART
 SM00985; UBA_e1_C 
PROSITE
 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 
PRINTS
 PR01849; UBIQUITINACT.