UniProt Accession

 ACON_RAT; Q9ER34; Q6P6V3 

Genbank Protein ID

 AJ243266; BC061999 

Genbank Nucleotide ID

 CAC11018.1; AAH61999.1 

Protein Name

 Aconitate hydratase, mitochondrial 

Protein Synonyms/Alias

 Aconitase; Citrate hydro-lyase 

Gene Name

 Aco2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
50	IRYDLLEKNINIVRK	acetylation	[1]
68	RPLTLSEKIVYGHLD	acetylation	[1]
86	NQEIERGKTYLRLRP	acetylation	[1]
138	EAQLGGEKDLRRAKD	acetylation	[1]
144	EKDLRRAKDINQEVY	acetylation	[1]
228	PWELKCPKVIGVKLT	acetylation	[1]
233	CPKVIGVKLTGTLSG	acetylation	[1]
245	LSGWTSPKDVILKVA	acetylation	[1]
304	FPYNHRMKKYLSKTG	acetylation	[1]
305	PYNHRMKKYLSKTGR	acetylation	[1]
309	RMKKYLSKTGRADIA	acetylation	[1]
401	GRSAAVAKQALAHGL	acetylation	[1]
462	WDRKDIKKGEKNTIV	acetylation	[1]
517	ETDFLTGKDGKKFKL	acetylation	[1]
520	FLTGKDGKKFKLEAP	acetylation	[1]
521	LTGKDGKKFKLEAPD	acetylation	[1]
523	GKDGKKFKLEAPDAD	acetylation	[1]
549	DTYQHPPKDSSGQRV	acetylation	[1]
573	QLLEPFDKWDGKDLE	acetylation	[1]
577	PFDKWDGKDLEDLQI	acetylation	[1]
605	SAAGPWLKFRGHLDN	acetylation	[1]
628	AINIENGKANSVRNA	acetylation	[1]
652	DTARYYKKHGIRWVV	acetylation	[1]
689	GGRAIITKSFARIHE	acetylation	[1]
700	RIHETNLKKQGLLPL	acetylation	[1]
717	ADPSDYNKIHPVDKL	acetylation	[1]
723	NKIHPVDKLTIQGLK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the isomerization of citrate to isocitrate via cis-aconitate (By similarity). 

Sequence Annotation

 REGION 192 194 Substrate binding (By similarity).
 REGION 670 671 Substrate binding (By similarity).
 METAL 385 385 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 448 448 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 451 451 Iron-sulfur (4Fe-4S) (By similarity).
 BINDING 99 99 Substrate (By similarity).
 BINDING 474 474 Substrate (By similarity).
 BINDING 479 479 Substrate (By similarity).
 BINDING 607 607 Substrate (By similarity).
 MOD_RES 28 28 Pyrrolidone carboxylic acid (By
 MOD_RES 50 50 N6-acetyllysine (By similarity).
 MOD_RES 559 559 Phosphoserine (By similarity).
 MOD_RES 573 573 N6-acetyllysine (By similarity).
 MOD_RES 605 605 N6-acetyllysine (By similarity).
 CROSSLNK 144 144 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 4Fe-4S; Acetylation; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Isopeptide bond; Lyase; Metal-binding; Mitochondrion; Phosphoprotein; Pyrrolidone carboxylic acid; Reference proteome; Transit peptide; Tricarboxylic acid cycle; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 780 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0051538; F:3 iron, 4 sulfur cluster binding; IDA:RGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:RGD.
 GO:0003994; F:aconitate hydratase activity; IDA:RGD.
 GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0005506; F:iron ion binding; IEA:Compara.
 GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0006101; P:citrate metabolic process; IDA:RGD.
 GO:0006102; P:isocitrate metabolic process; IDA:RGD.
 GO:0006099; P:tricarboxylic acid cycle; IDA:RGD. 

Interpro

 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS.
 IPR006248; Aconitase_mito-like.
 IPR000573; AconitaseA/IPMdHydase_ssu_swvl. 

Pfam

 PF00330; Aconitase
 PF00694; Aconitase_C 

SMART

  

PROSITE

 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 

PRINTS

 PR00415; ACONITASE.