CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029572
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 14-3-3 protein zeta/delta 
Protein Synonyms/Alias
 cDNA, FLJ79516, highly similar to 14-3-3 protein zeta/delta 
Gene Name
 YWHAZ 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAREYREKIETELRDacetylation[1]
8MAREYREKIETELRDubiquitination[1, 2]
38NASQAESKVFYLKMKacetylation[1, 3]
38NASQAESKVFYLKMKubiquitination[1, 2, 4, 5]
61EVAAGDDKKGIVDQSacetylation[1, 3]
61EVAAGDDKKGIVDQSubiquitination[1, 2, 5, 6, 7, 8, 9, 10]
62VAAGDDKKGIVDQSQubiquitination[1, 2, 4, 5, 6, 7, 8, 9]
80QEAFEISKKEMQPTHacetylation[1, 3]
80QEAFEISKKEMQPTHubiquitination[2, 5, 6, 7]
81EAFEISKKEMQPTHPacetylation[3]
81EAFEISKKEMQPTHPubiquitination[1, 2, 4, 5, 6, 7]
116EKACSLAKTAFDEAIubiquitination[7, 9]
135TLSEESYKDSTLIMQmethylation[11]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [11] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 168 AA 
Protein Sequence
MAREYREKIE TELRDICNDV LSLLEKFLIP NASQAESKVF YLKMKGDYYR YLAEVAAGDD 60
KKGIVDQSQQ AYQEAFEISK KEMQPTHPIR LGLALNFSVF YYEILNSPEK ACSLAKTAFD 120
EAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDTQGDEA EAGEGGEN 168 
Gene Ontology
 GO:0031252; C:cell leading edge; IEA:Compara.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0002553; P:histamine secretion by mast cell; IEA:Compara.
 GO:0006626; P:protein targeting to mitochondrion; IEA:Compara.
 GO:0010941; P:regulation of cell death; IEA:Compara. 
Interpro
 IPR000308; 14-3-3.
 IPR023409; 14-3-3_CS.
 IPR023410; 14-3-3_domain. 
Pfam
 PF00244; 14-3-3 
SMART
 SM00101; 14_3_3 
PROSITE
 PS00797; 1433_2 
PRINTS
 PR00305; 1433ZETA.