UniProt Accession

 NAP1_YEAST; P25293; D6VXA9; P87196 

Genbank Protein ID

 M63555; AY692777; Z28272; Z28273; BK006944 

Genbank Nucleotide ID

 AAA34811.1; AAT92796.1; CAA82124.2; CAA82125.1; DAA09199.1 

Protein Name

 Nucleosome assembly protein 

Protein Synonyms/Alias

  

Gene Name

 NAP1 

Gene Synonyms/Alias

 YKR048C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
50	QAQEQDDKIGTINEE	ubiquitination	[1, 2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Acidic protein, which assembles histones into an octamer (in vitro). Involved in the regulation of the localization and the function of the septins during mitosis. Involved in the function of B-type cyclins. 

Sequence Annotation

 DNA_BIND 330 356 H-T-H motif (Potential).
 REGION 143 362 Interaction with NBA1.
 MOD_RES 20 20 Phosphothreonine.
 MOD_RES 24 24 Phosphothreonine.
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 53 53 Phosphothreonine.
 MOD_RES 69 69 Phosphoserine.
 MOD_RES 76 76 Phosphoserine.
 MOD_RES 82 82 Phosphoserine.
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 104 104 Phosphoserine.
 MOD_RES 140 140 Phosphoserine.
 MOD_RES 159 159 Phosphoserine; by CK2.
 MOD_RES 177 177 Phosphoserine; by CK2.
 MOD_RES 397 397 Phosphoserine; by CK2.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 417 AA 

Protein Sequence

Gene Ontology

 GO:0005935; C:cellular bud neck; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IPI:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0042393; F:histone binding; IDA:SGD.
 GO:0007117; P:budding cell bud growth; IGI:SGD.
 GO:0006334; P:nucleosome assembly; IDA:SGD.
 GO:0006337; P:nucleosome disassembly; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0006606; P:protein import into nucleus; IMP:SGD. 

Interpro

 IPR002164; NAP_family. 

Pfam

 PF00956; NAP 

SMART

  

PROSITE

  

PRINTS