UniProt Accession

 CD47_HUMAN; Q08722; A8K198; D3DN59; Q53Y71; Q96A60 

Genbank Protein ID

 X69398; Z25521; BT006907; AK289813; CH471052; CH471052; BC010016; BC012884; BC037306 

Genbank Nucleotide ID

 CAA49196.1; CAA80977.1; AAP35553.1; BAF82502.1; EAW79733.1; EAW79734.1; AAH10016.1; AAH12884.1; AAH37306.1 

Protein Name

 Leukocyte surface antigen CD47 

Protein Synonyms/Alias

 Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47 

Gene Name

 CD47 

Gene Synonyms/Alias

 MER6 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
74	TFDGALNKSTVPTDF	ubiquitination	[1, 2]
85	PTDFSSAKIEVSQLL	ubiquitination	[2, 3]
93	IEVSQLLKGDASLKM	ubiquitination	[2]
99	LKGDASLKMDKSDAV	ubiquitination	[1, 2]
102	DASLKMDKSDAVSHT	ubiquitination	[2]
314	EEPLNAFKESKGMMN	ubiquitination	[1, 2, 4]
317	LNAFKESKGMMNDE*	ubiquitination	[1, 2, 4, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 

Sequence Annotation

 DOMAIN 19 127 Ig-like V-type.
 MOD_RES 19 19 Pyrrolidone carboxylic acid.
 CARBOHYD 23 23 N-linked (GlcNAc...).
 CARBOHYD 34 34 N-linked (GlcNAc...).
 CARBOHYD 50 50 N-linked (GlcNAc...).
 CARBOHYD 73 73 N-linked (GlcNAc...).
 CARBOHYD 111 111 N-linked (GlcNAc...).
 CARBOHYD 206 206 N-linked (GlcNAc...) (Potential).
 DISULFID 33 263
 DISULFID 41 114  

Keyword

 3D-structure; Alternative splicing; Cell adhesion; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Pyrrolidone carboxylic acid; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0070053; F:thrombospondin receptor activity; IPI:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0007229; P:integrin-mediated signaling pathway; TAS:ProtInc.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0008228; P:opsonization; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB.
 GO:0022409; P:positive regulation of cell-cell adhesion; IDA:UniProtKB.
 GO:0050729; P:positive regulation of inflammatory response; IEA:Compara.
 GO:0050766; P:positive regulation of phagocytosis; IEA:Compara.
 GO:0050870; P:positive regulation of T cell activation; IDA:UniProtKB.
 GO:0009617; P:response to bacterium; IEA:Compara. 

Interpro

 IPR006704; CD47.
 IPR013147; CD47_TM.
 IPR013270; CD47_Vset.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold. 

Pfam

 PF04549; CD47
 PF08204; V-set_CD47 

SMART

  

PROSITE

 PS50835; IG_LIKE 

PRINTS