CPLM-020866

Genbank Nucleotide ID

Charged multivesicular body protein 4b

Protein Synonyms/Alias

Chromatin-modifying protein 4b; CHMP4b

Mus musculus (Mouse)

Position	Peptide	Type	References
6	**MSVFGKLFGAGGG	acetylation	[1, 2, 3]
107	NTNTEVLKNMGYAAK	ubiquitination	[4]
114	KNMGYAAKAMKAAHD	acetylation	[1]

[1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding (By similarity).

REGION 2 153 Intramolecular interaction with C-
REGION 154 224 Intramolecular interaction with N-
MOD_RES 2 2 N-acetylserine (By similarity).
MOD_RES 6 6 N6-acetyllysine (By similarity).
MOD_RES 114 114 N6-acetyllysine (By similarity).
MOD_RES 184 184 Phosphoserine (By similarity).
MOD_RES 223 223 Phosphoserine (By similarity).

Acetylation; Coiled coil; Complete proteome; Cytoplasm; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.