UniProt Accession

 DX39B_HUMAN; Q13838; B0S8C0; O43496; Q0EFA1; Q2L6F9; Q53GL9; Q5RJ64; Q5RJ66; Q5ST94; Q5STB4; Q5STB5; Q5STB7; Q5STB8; Q5STU4; Q5STU5; Q5STU6; Q5STU8; Q71V76 

Genbank Protein ID

 Z37166; BT009909; AK222912; AB088115; AB103621; AB202112; BA000025; AL662847; AL662847; AL662801; AL662801; AL662801; AL662801; AL662801; BX001040; BX248516; BX927320; CR753820; CR753864; CH471081; BC000361; BC013006; AF029061; AF029062 

Genbank Nucleotide ID

 CAA85523.1; AAP88911.1; BAD96632.1; BAC54953.1; BAF31287.1; BAE78637.1; BAB63306.1; CAI17664.2; CAI17665.2; CAI18279.2; CAI18280.1; CAI18281.2; CAI18282.2; CAI18283.2; CAI18634.1; CAI41922.1; CAQ09974.1; CAQ07176.1; CAQ10634.1; EAX03404.1; AAH00361.1; AAH13006.1; AAB94615.1; AAC63046.1 

Protein Name

 Spliceosome RNA helicase DDX39B 

Protein Synonyms/Alias

 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein 

Gene Name

 DDX39B 

Gene Synonyms/Alias

 BAT1; UAP56 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	GAEAPAKKDVKGSYV	ubiquitination	[1]
36	APAKKDVKGSYVSIH	acetylation	[2]
36	APAKKDVKGSYVSIH	ubiquitination	[1]
95	QAKSGMGKTAVFVLA	ubiquitination	[1]
138	KEYERFSKYMPNVKV	ubiquitination	[1]
188	RNKSLNLKHIKHFIL	acetylation	[2]
256	IFVDDETKLTLHGLQ	ubiquitination	[1, 3]
268	GLQQYYVKLKDNEKN	ubiquitination	[1]
334	LSRYQQFKDFQRRIL	ubiquitination	[1]
384	RAGRFGTKGLAITFV	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. 

Sequence Annotation

 DOMAIN 76 249 Helicase ATP-binding.
 DOMAIN 261 422 Helicase C-terminal.
 NP_BIND 89 96 ATP.
 MOTIF 45 73 Q motif.
 MOTIF 196 199 DECD box.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 36 36 N6-acetyllysine.
 MOD_RES 38 38 Phosphoserine.
 MOD_RES 172 172 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; mRNA processing; mRNA splicing; mRNA transport; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 428 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IDA:BHF-UCL.
 GO:0000346; C:transcription export complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043008; F:ATP-dependent protein binding; IDA:BHF-UCL.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:UniProtKB.
 GO:0030621; F:U4 snRNA binding; IDA:BHF-UCL.
 GO:0017070; F:U6 snRNA binding; IDA:BHF-UCL.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IDA:UniProtKB.
 GO:0010501; P:RNA secondary structure unwinding; IDA:BHF-UCL.
 GO:0000245; P:spliceosomal complex assembly; IDA:BHF-UCL. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS