CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006092
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein NUD1 
Protein Synonyms/Alias
  
Gene Name
 NUD1 
Gene Synonyms/Alias
 YOR373W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
35NAHSNSGKVFKEYESacetylation[1]
149VLETNTFKRHLDMKNubiquitination[2]
246NSININNKDNARTKEubiquitination[2]
357APSILDKKAYELTSTubiquitination[2, 3]
365AYELTSTKPGDVGYRubiquitination[2]
375DVGYRQKKIQEEENLubiquitination[2]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Involved in astral microtubule organization by binding SCP72 to the outer plaque in a cell-cycle dependent manner. Required for the mitotic exit by facilitating the binding of TEMP1 to CDC15. Also involved in the pathway that organizes the shaping and sizing of the prospore membrane (PSM) during sporulation. 
Sequence Annotation
 REPEAT 544 566 LRR 1.
 REPEAT 567 588 LRR 2.
 REPEAT 589 609 LRR 3.
 REPEAT 621 642 LRR 4.
 REPEAT 643 664 LRR 5.
 MOD_RES 388 388 Phosphothreonine.
 MOD_RES 392 392 Phosphothreonine.  
Keyword
 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Leucine-rich repeat; Meiosis; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 851 AA 
Protein Sequence
MDMDTQEAEL SSQLENLTIN SPRKLRSNAH SNSGKVFKEY ESNHDFQDSN FTSQVVEPAI 60
SDSVKKPPTM TVLNNYSTVH QKVPSGFSGT TATSHQEAQW KQYFPGIGSG GGTNFGGAVG 120
TANKVPESDL IVSDLVKDLS GVLETNTFKR HLDMKNKTTT MQTHENHDTI SISHSKDFFN 180
AEKVSSSFSD DSDSGPAAEA HDVFDGILQK QKSNYLVGSY PSNSNNKNNN NNNNNNNNNS 240
ININNKDNAR TKEEDEEDTS NSFEFSSSSS MSSSQTQSGR KSKVLKKPPL NTISPGQLGY 300
QFNHTHGAWD PPLNQGLDVS SSHSLDNTSS NQSQFATMVP TGDNHTNGKA PSILDKKAYE 360
LTSTKPGDVG YRQKKIQEEE NLANSDDTPL DTPKFNDLFT KNGTRAKVKG QMRTSRSISN 420
SNLLEAHKKL KTFPAERVED ITSISEVNTS FNETEKQLIS ILTSKLSGSP SYDSDWEKIL 480
KVDLSRGKLK NMFGMQRLLP NVLVLNLSDN EMNTLEGIPS NVVQLFCSNN KITSAHCSLA 540
GFHDLECLDL SYNLLNTSLK FLSLCHHLQE VNLSYNSIQS LEGIGSSRMK KLNLSNNEIN 600
GIIDFEQLIL TNNSVVGGWL TVEVLDLSNN NIIGVRNINC LPRLKVLNLN GNPLVSIVES 660
SKMENGTLRA LSIKNTGGAL SKLQNYKLDD QFTFPYQNLK ILKLDGFAQL SKWQKWPATL 720
QILEINGGLA SSLPRFSSLK STNLYSLTIA NVRDFTHLPV DLSKELPFLQ ELHLPGNNLQ 780
NAHKLTKTLP RQSVKFLDLR NNPITTPRHD RASTSLHYRQ LLQLAGLCQQ QCPALATLWL 840
DDTPAPTATN L 851 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005200; F:structural constituent of cytoskeleton; IPI:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051293; P:establishment of spindle localization; IMP:SGD.
 GO:0010458; P:exit from mitosis; IMP:SGD.
 GO:0045132; P:meiotic chromosome segregation; IMP:SGD.
 GO:0000073; P:spindle pole body separation; IMP:SGD. 
Interpro
 IPR001611; Leu-rich_rpt. 
Pfam
  
SMART
  
PROSITE
 PS51450; LRR 
PRINTS