CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030252
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase 
Protein Synonyms/Alias
 Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase, isoform CRA_c; DNA fragmentation factor subunit beta 
Gene Name
 Cad 
Gene Synonyms/Alias
 mCG_23490 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
186CALDCGLKYNQIRCLacetylation[1]
696RSSALASKATGYPLAacetylation[2]
696RSSALASKATGYPLAubiquitination[3]
760TKIGSCMKSVGEVMGubiquitination[3]
778SFEEAFQKALRMVDEubiquitination[3]
867QDLLHQAKCLGFSDKubiquitination[3]
1228RSFPFVSKTLGVDLVacetylation[2]
1246TRIIMGEKVEPVGLMacetylation[4]
1309AMLSTGFKIPEKNILacetylation[2, 5]
1309AMLSTGFKIPEKNILsuccinylation[5]
1325TIGSYKNKSELLPTVacetylation[5]
1325TIGSYKNKSELLPTVsuccinylation[5]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2225 AA 
Protein Sequence
MAALVLEDGS VLQGRPFGAA VSTAGEVVFQ TGMVGYPEAL TDPSYKAQIL VLTYPLIGNY 60
GIPSDEEDEF GLSKWFESSE IHVAGLVVGE CCPTPSHWSA NCTLHEWLQQ RGIPGLQGVD 120
TRELTKKLRE QGSLLGKLVQ KGTEPSALPF VDPNARPLAP EVSIKTPRVF NAGGAPRICA 180
LDCGLKYNQI RCLCQLGAEV TVVPWDHELD SQKYDGLFLS NGPGDPASYP GVVSTLSRVL 240
SEPNPRPVFG ICLGHQLLAL AIGAKTYKMR YGNRGHNQPC LLVGTGRCFL TSQNHGFAVD 300
ADSLPAGWAP LFTNANDCSN EGIVHDSLPF FSVQFHPEHR AGPSDMELLF DVFLETVREA 360
AAGNIGGQTV RERLAQRLCP PELPIPGSGL PPPRKVLILG SGGLSIGQAG EFDYSGSQAI 420
KALKEENIQT LLINPNIATV QTSQGLADKV YFLPITLHYV TQVIRNERPD GVLLTFGGQT 480
ALNCGVELTK AGVLARYGVR VLGTPVETIE LTEDRRAFAA RMAEIGEHVA PSEAANSLEQ 540
AQAAAERLGY PVLVRAAFAL GGLGSGFAST KEELSALVAP AFAHTSQVLI DKSLKGWKEI 600
EYEVVRDAYG NCVTVCNMEN LDPLGIHTGE SIVVAPSQTL NDREYQLLRR TAIKVTQHLG 660
IVGECNVQYA LNPESEQYYI IEVNARLSRS SALASKATGY PLAYVAAKLA LGIPLPELRN 720
SVTGGTAAFE PSLDYCVVKI PRWDLSKFLR VSTKIGSCMK SVGEVMGIGR SFEEAFQKAL 780
RMVDENCVGF DHTVKPVSDM ELETPTDKRI FVVAAALWAG YSVERLYELT RIDCWFLHRM 840
KRIVTHAQLL EQHRGQALPQ DLLHQAKCLG FSDKQIALAV LSTELAVRKL RQELGICPAV 900
KQIDTVAAEW PAQTNYLYLT YWGNTHDLDF RAPHVLVLGS GVYRIGSSVE FDWCAVGCIQ 960
QLRKMGYKTI MVNYNPETVS TDYDMCDRLY FDEISFEVVM DIYELENPEG VILSMGGQLP 1020
NNMAMALHRQ QCRVLGTSPE AIDSAENRFK FSRLLDTIGI SQPQWRELSD LESARQFCHT 1080
VGYPCVVRPS YVLSGAAMNV AYTDGDLERF LSSAAAVSKE HPVVISKFIQ EAKEIDVDAV 1140
ACDGIVSAIA ISEHVENAGV HSGDATLVTP PQDITPKTLE RIKAIVHAVG QELQVTGPFN 1200
LQLIAKDDQL KVIECNVRVS RSFPFVSKTL GVDLVALATR IIMGEKVEPV GLMTGSGVVG 1260
VKVPQFSFSR LAGADVVLGV EMTSTGEVAG FGESRCEAYL KAMLSTGFKI PEKNILLTIG 1320
SYKNKSELLP TVRLLESLGY SLYASLGTAD FYTEHGVKVT AVDWHFEEAV DGECPPQRSI 1380
LDQLAENHFE LVINLSMRGA GGRRLSSFVT KGYRTRRLAA DFSVPLIIDI KCTKLFVEAL 1440
GQIGPAPPLK VHVDCMTSQK LVRLPGLIDV HVHLREPGGT HKEDFASGTA AALAGGVTMV 1500
CAMPNTRPPI IDAPALALAQ KLAEAGARCD FTLFLGASSE NAGTLGAVAG SAAGLKLYLN 1560
ETFSELRLDS VAQWMEHFET WPAHLPIVAH AERQSVAAVL MVAQLTQRPV HICHVARKEE 1620
ILLIKTAKAQ GLPVTCEVAP HHLFLNREDL ERLGPGKGEV RPELGSREDM EALWENMAVI 1680
DCFASDHAPH TLEEKCGPKP PPGFPGLETM LPLLLTAVSE GRLSLDDLLQ RLHHNPRRIF 1740
HLPLQEDTYV EVDLEHEWTV PSHMPFSKAR WTPFEGQKVK GTVRRVVLRG EVAYIDGQVL 1800
VPPGYGQDVR KWPQGVVPQP PPSTPATTEI TTTPERPRRV IPGLPDGRFH LPPRIHRASD 1860
PGLPAEEPKE KPPRKVVEPE LMGTPDGPCY PAPPVPRQAS PQNLGSSGLL HPQMSPLLHS 1920
LVGQHILSVK QFTKDQMSHL FNVAHTLRMM VQKERSLDIL KGKVMASMFY EVSTRTSSSF 1980
AAAMARLGGA VLSFSEATSS VQKGESLADS VQTMSCYADV IVLRHPQPGA VELAAKHCRR 2040
PVINAGDGVG EHPTQALLDI FTIREELGTV NGMTITMVGD LKHGRTVHSL ACLLTQYRVS 2100
LRYVAPPSLR MPPSVRDFVA SRGTKQEEFE SIEEALPDTD VLYMTRIQKE RFGSVQEYEA 2160
CFGQFILTPH IMTRAKKKMV VMHPMPRVNE ISVEVDSDPR AAYFRQAENG MYIRMALLAT 2220
VLGRF 2225 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0016363; C:nuclear matrix; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0043195; C:terminal bouton; IEA:Compara.
 GO:0016597; F:amino acid binding; IEA:InterPro.
 GO:0004070; F:aspartate carbamoyltransferase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IEA:Compara.
 GO:0004151; F:dihydroorotase activity; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:Compara.
 GO:0070409; P:carbamoyl phosphate biosynthetic process; IEA:InterPro.
 GO:0035690; P:cellular response to drug; IEA:Compara.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; IEA:Compara.
 GO:0017144; P:drug metabolic process; IEA:Compara.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0006543; P:glutamine catabolic process; IEA:InterPro.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0014075; P:response to amine stimulus; IEA:Compara.
 GO:0031000; P:response to caffeine; IEA:Compara.
 GO:0051414; P:response to cortisol stimulus; IEA:Compara.
 GO:0033574; P:response to testosterone stimulus; IEA:Compara.
 GO:0006228; P:UTP biosynthetic process; IEA:Compara. 
Interpro
 IPR006132; Asp/Orn_carbamoyltranf_P-bd.
 IPR006130; Asp/Orn_carbamoylTrfase.
 IPR002082; Asp_carbamoyltransf.
 IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR006275; CarbamoylP_synth_lsu.
 IPR005481; CarbamoylP_synth_lsu_N.
 IPR005480; CarbamoylP_synth_lsu_oligo.
 IPR006274; CarbamoylP_synth_ssu.
 IPR002474; CarbamoylP_synth_ssu_N.
 IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
 IPR005483; CbamoylP_synth_lsu_CPSase_dom.
 IPR002195; Dihydroorotase_CS.
 IPR017926; GATASE.
 IPR011059; Metal-dep_hydrolase_composite.
 IPR011607; MGS-like_dom.
 IPR016185; PreATP-grasp_dom. 
Pfam
 PF00289; CPSase_L_chain
 PF02786; CPSase_L_D2
 PF02787; CPSase_L_D3
 PF00988; CPSase_sm_chain
 PF00117; GATase
 PF02142; MGS
 PF00185; OTCace
 PF02729; OTCace_N 
SMART
 SM01096; CPSase_L_D3
 SM01097; CPSase_sm_chain
 SM00851; MGS 
PROSITE
 PS50975; ATP_GRASP
 PS00097; CARBAMOYLTRANSFERASE
 PS00866; CPSASE_1
 PS00867; CPSASE_2
 PS00482; DIHYDROOROTASE_1
 PS00483; DIHYDROOROTASE_2
 PS51273; GATASE_TYPE_1 
PRINTS
 PR00100; AOTCASE.
 PR00101; ATCASE.
 PR00098; CPSASE.