CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015126
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endoplasmic reticulum aminopeptidase 2 
Protein Synonyms/Alias
 Leukocyte-derived arginine aminopeptidase; L-RAP 
Gene Name
 ERAP2 
Gene Synonyms/Alias
 LRAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
135SRYMKPGKELKVLSYacetylation[1]
138MKPGKELKVLSYPAHacetylation[1]
138MKPGKELKVLSYPAHubiquitination[2]
155IALLVPEKLTPHLKYubiquitination[2]
181DGFEGFYKSTYRTLGubiquitination[2]
484GIIQYLKKFSYRNAKubiquitination[2]
491KFSYRNAKNDDLWSSubiquitination[2]
545MTTWTLQKGIPLLVVubiquitination[2]
553GIPLLVVKQDGCSLRubiquitination[2]
608HRHILKSKTDTLDLPubiquitination[2]
731RYLLQYFKPVIDRQSubiquitination[2]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys. 
Sequence Annotation
 REGION 334 338 Substrate binding.
 ACT_SITE 371 371 Proton acceptor.
 METAL 370 370 Zinc; catalytic.
 METAL 374 374 Zinc; catalytic.
 METAL 393 393 Zinc; catalytic.
 BINDING 200 200 Substrate.
 CARBOHYD 85 85 N-linked (GlcNAc...).
 CARBOHYD 119 119 N-linked (GlcNAc...).
 CARBOHYD 405 405 N-linked (GlcNAc...).
 CARBOHYD 650 650 N-linked (GlcNAc...).
 DISULFID 421 460
 DISULFID 759 766  
Keyword
 3D-structure; Adaptive immunity; Alternative splicing; Aminopeptidase; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Hydrolase; Immunity; Membrane; Metal-binding; Metalloprotease; Polymorphism; Protease; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 960 AA 
Protein Sequence
MFHSSAMVNS HRKPMFNIHR GFYCLTAILP QICICSQFSV PSSYHFTEDP GAFPVATNGE 60
RFPWQELRLP SVVIPLHYDL FVHPNLTSLD FVASEKIEVL VSNATQFIIL HSKDLEITNA 120
TLQSEEDSRY MKPGKELKVL SYPAHEQIAL LVPEKLTPHL KYYVAMDFQA KLGDGFEGFY 180
KSTYRTLGGE TRILAVTDFE PTQARMAFPC FDEPLFKANF SIKIRRESRH IALSNMPKVK 240
TIELEGGLLE DHFETTVKMS TYLVAYIVCD FHSLSGFTSS GVKVSIYASP DKRNQTHYAL 300
QASLKLLDFY EKYFDIYYPL SKLDLIAIPD FAPGAMENWG LITYRETSLL FDPKTSSASD 360
KLWVTRVIAH ELAHQWFGNL VTMEWWNDIW LKEGFAKYME LIAVNATYPE LQFDDYFLNV 420
CFEVITKDSL NSSRPISKPA ETPTQIQEMF DEVSYNKGAC ILNMLKDFLG EEKFQKGIIQ 480
YLKKFSYRNA KNDDLWSSLS NSCLESDFTS GGVCHSDPKM TSNMLAFLGE NAEVKEMMTT 540
WTLQKGIPLL VVKQDGCSLR LQQERFLQGV FQEDPEWRAL QERYLWHIPL TYSTSSSNVI 600
HRHILKSKTD TLDLPEKTSW VKFNVDSNGY YIVHYEGHGW DQLITQLNQN HTLLRPKDRV 660
GLIHDVFQLV GAGRLTLDKA LDMTYYLQHE TSSPALLEGL SYLESFYHMM DRRNISDISE 720
NLKRYLLQYF KPVIDRQSWS DKGSVWDRML RSALLKLACD LNHAPCIQKA AELFSQWMES 780
SGKLNIPTDV LKIVYSVGAQ TTAGWNYLLE QYELSMSSAE QNKILYALST SKHQEKLLKL 840
IELGMEGKVI KTQNLAALLH AIARRPKGQQ LAWDFVRENW THLLKKFDLG SYDIRMIISG 900
TTAHFSSKDK LQEVKLFFES LEAQGSHLDI FQTVLETITK NIKWLEKNLP TLRTWLMVNT 960 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; TAS:HGNC.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004177; F:aminopeptidase activity; IDA:HGNC.
 GO:0008237; F:metallopeptidase activity; IDA:HGNC.
 GO:0008270; F:zinc ion binding; TAS:HGNC.
 GO:0019885; P:antigen processing and presentation of endogenous peptide antigen via MHC class I; TAS:HGNC.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0008217; P:regulation of blood pressure; TAS:HGNC. 
Interpro
 IPR024571; DUF3358.
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N. 
Pfam
 PF11838; DUF3358
 PF01433; Peptidase_M1 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00756; ALADIPTASE.