UniProt Accession

 IDH3A_MOUSE; Q9D6R2; Q3UAM8; Q8C8A1; Q9D1L1 

Genbank Protein ID

 AK003393; AK010065; AK032787; AK047951; AK150618; AK151304; AK152353; AK153459; AK158646; AK159051; AK168049; AK168149; AK169152; BC034273; BC049956 

Genbank Nucleotide ID

 BAB22760.1; BAB26679.1; BAC28021.1; BAC33199.1; BAE29708.1; BAE30286.1; BAE31145.1; BAE32011.1; BAE34596.1; BAE34785.1; BAE40031.1; BAE40114.1; BAE40931.1; AAH34273.1; AAH49956.1 

Protein Name

 Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial 

Protein Synonyms/Alias

 Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha 

Gene Name

 Idh3a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
58	MKIFDAAKAPIQWEE	acetylation	[1, 2, 3, 4, 5]
58	MKIFDAAKAPIQWEE	succinylation	[4]
58	MKIFDAAKAPIQWEE	ubiquitination	[6]
77	AIQGPGGKWMIPPEA	acetylation	[1, 2, 3, 4, 5, 7]
77	AIQGPGGKWMIPPEA	succinylation	[4]
85	WMIPPEAKESMDKNK	acetylation	[5]
90	EAKESMDKNKMGLKG	acetylation	[3]
100	MGLKGPLKTPIAAGH	acetylation	[2, 4, 8]
100	MGLKGPLKTPIAAGH	succinylation	[4]
100	MGLKGPLKTPIAAGH	ubiquitination	[6]
177	LITEEASKRIAEFAF	acetylation	[7]
177	LITEEASKRIAEFAF	ubiquitination	[6]
200	SNVTAVHKANIMRMS	acetylation	[2, 4]
200	SNVTAVHKANIMRMS	succinylation	[4]
200	SNVTAVHKANIMRMS	ubiquitination	[6]
214	SDGLFLQKCREVAEN	acetylation	[2]
223	REVAENCKDIKFNEM	acetylation	[3, 5, 8]
336	AACFATIKDGKSLTK	acetylation	[1, 2, 3, 4, 5]
336	AACFATIKDGKSLTK	succinylation	[4]
339	FATIKDGKSLTKDLG	acetylation	[3]
343	KDGKSLTKDLGGNAK	acetylation	[2, 3, 4]
343	KDGKSLTKDLGGNAK	succinylation	[4]
343	KDGKSLTKDLGGNAK	ubiquitination	[6]
350	KDLGGNAKCSDFTEE	acetylation	[3, 4]
350	KDLGGNAKCSDFTEE	succinylation	[4]

Reference

 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [8] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

  

Sequence Annotation

 METAL 233 233 Magnesium or manganese (By similarity).
 METAL 257 257 Magnesium or manganese (By similarity).
 METAL 261 261 Magnesium or manganese (By similarity).
 BINDING 115 115 Substrate (By similarity).
 BINDING 125 125 Substrate (By similarity).
 BINDING 146 146 Substrate (By similarity).
 BINDING 233 233 Substrate (By similarity).
 MOD_RES 343 343 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 366 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR004434; Isocitrate_DH_NAD.
 IPR024084; IsoPropMal-DH-like_dom. 

Pfam

 PF00180; Iso_dh 

SMART

  

PROSITE

 PS00470; IDH_IMDH 

PRINTS