CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017163
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-CoA dehydrogenase family member 9, mitochondrial 
Protein Synonyms/Alias
 ACAD-9 
Gene Name
 Acad9 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
45LQSCSFAKELFLGNIacetylation[1]
96RKIDQEGKIPVDTLEacetylation[2, 3]
96RKIDQEGKIPVDTLEsuccinylation[3]
104IPVDTLEKLKSLGLFacetylation[2, 3]
104IPVDTLEKLKSLGLFsuccinylation[3]
206RATLSEDKKYFILNGacetylation[2, 4]
242VVDSDGSKTDKMTAFacetylation[2, 3, 4]
242VVDSDGSKTDKMTAFsuccinylation[3]
245SDGSKTDKMTAFIVEacetylation[2]
525TLLLRFGKNIVEEQLacetylation[1, 2, 3, 5]
525TLLLRFGKNIVEEQLsuccinylation[3]
612VSRQILEKRAYICAHacetylation[2, 4]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758
Functional Description
 Has a dehydrogenase activity on palmitoyl-CoA (C16:0) and stearoyl-CoA (C18:0). It is three times more active on palmitoyl-CoA then on stearoyl-CoA. Has little activity on octanoyl-CoA (C8:0), butyryl-CoA (C4:0) or isovaleryl-CoA (5:0) (By similarity). 
Sequence Annotation
 ACT_SITE 430 430 Proton acceptor (By similarity).
 MOD_RES 45 45 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 625 AA 
Protein Sequence
MSGCVLLSRG ATAAAAAARA SRVLREFTAR RRPLHTSLQS CSFAKELFLG NIEQKGVFPF 60
PEVSQHELSE INQFVGPLEK FFTEEVDSRK IDQEGKIPVD TLEKLKSLGL FGIQVPEEYG 120
GLGLSNTMYA RLGEIISLDA SITVTLAAHQ AIGLKGIILV GNEEQKAKYL PKLSSGEHIA 180
AFCLTEPASG SDAASIQTRA TLSEDKKYFI LNGSKVWITN GGLANIFTVF AKTEVVDSDG 240
SKTDKMTAFI VERDFGGITN GKPEDKLGIR GSNTCEVHFE NTRVPVENVL GEVGGGFKVA 300
MNILNSGRFS MGSAVAGMLK KLIELTAEYA CTRKQFNRNL SEFGLIQEKF ALMAQKAYVM 360
ESMAYLTSGM LDQPGFPDCS IEAAMVKVFS SEAAWQCVSE ALQILGGSGY MKDYPYERML 420
RDARILLIFE GTNEILRLFI ALTGLQHAGR ILTSRIKELK SGNVTTVMET IGRKLRDSLG 480
RTVDLGLTGD LGVVHPSLGD SANKLEENVH YFGRTVETLL LRFGKNIVEE QLVLKRVANI 540
LINLYGMTAV LSRASRSIRI GLRNHDHEVL LANMFCVEAY FQNLFSLSQL DKNAPENLDE 600
QIKKVSRQIL EKRAYICAHP LDRAS 625 
Gene Ontology
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro. 
Interpro
 IPR006089; Acyl-CoA_DH_CS.
 IPR006092; Acyl-CoA_DH_N.
 IPR006090; Acyl-CoA_Oxase/DH_1.
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 
Pfam
 PF00441; Acyl-CoA_dh_1
 PF02770; Acyl-CoA_dh_M
 PF02771; Acyl-CoA_dh_N 
SMART
  
PROSITE
 PS00072; ACYL_COA_DH_1
 PS00073; ACYL_COA_DH_2 
PRINTS